摘要
为探究干旱胁迫和硝铵比对烟苗假植期间叶绿素、渗透调节物质和抗氧化酶活性的影响,采用水旱两段式育苗方法,研究硝铵比为100∶0、50∶50、0∶100时分别在正常水分、轻度干旱及重度干旱下烟苗叶片中叶绿素、丙二醛(MDA)含量及超氧化物岐化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性的变化规律。结果表明:烟苗经干旱处理后,随着干旱程度的加重,叶绿素含量呈先缓慢下降后迅速下降的趋势;MDA含量呈先缓慢上升后迅速下降的趋势;SOD、POD、CAT活性呈上升趋势,但重度干旱后期呈不同程度的下降。在3种硝态氮和铵态氮配比下,均为硝铵混合处理显著优于单一硝态氮源或单一铵态氮源。综合而言,在不同程度的干旱逆境下,硝铵混合营养较单一硝态氮或单一铵态氮更有利于提高假植期间烟苗的抗旱性,其中在轻度干旱胁迫条件下,硝铵混合营养最有利于烟苗生长,从而维持较好的抗旱性。
The effects of drought stress and different ratios of nitrate and ammonium nitrogen on chlorophyll, osmotic adjustment and antioxidant enzymes activities during the provisonal planting period of tobacco seedlings were studied, in order to provide reference for the growth regulation of tobacco seedlings in dry periods. The chlorophyll content, malondialdehyde content, SOD activity, POD activity and CAT activity in tobacco seedlings with the different ratio of nitrate and ammonium nitrogen of 100∶0, 50∶50 and 0∶100 in normal moisture content, mild drought and severe drought were measured. The results showed that after drought treatment, the chlorophyll content of tobacco decreased slowly and then decreased rapidly with the increase of drought degree;MDA content increased slowly and then decreased rapidly;SOD, POD and CAT activities increased at first, however, it showed different degrees of decline in the late stage of severe drought. Under the three different ratios of nitrate and ammonium nitrogen, the results of mixed nitrate and ammonium nitrate nutrition treatment were significantly better than single nitrate nitrogen source or single ammonium nitrogen source(P<0.05). Under different degrees of drought stress, mixed nitrate and ammonium nutrition was more effective than single nitrate nitrogen and single ammonium nitrogen in improving the drought resistance of tobacco during the provisonal planting period. Under mild drought stress, mixed nitrate and ammonium nutrition was most beneficial to the growth of tobacco seedlings, thus maintaining better drought resistance.
作者
刘璐
李强
丁梦娇
秦缘
周冀衡
LIU Lu;LI Qiang;DING Mengjiao;QIN Yuan;ZHOU Jiheng(College of Bioscience and Technology,Hunan Agricultural University,Changsha 410128,China;Institute of Tobacco,Hunan Agricultural University,Changsha 410128,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2019年第5期117-122,共6页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
湖南中烟工业有限责任公司科研项目(201843000934131)
关键词
烤烟
干旱胁迫
氮素形态
叶绿素
丙二醛
抗氧化酶活性
tobacco
drought stress
nitrogen form
chlorophyll
malondialdehyde
antioxidant enzyme activity
