摘要
目的 观察蛴螬提取物对视网膜中央静脉阻塞模型兔视神经小胶质细胞 CD40 表达的影响.方法 将60只有色大兔按随机数字表法分为空白组、模型组、血栓通组及蛴螬组,血栓通组灌胃复方血栓通片混悬液5 mg/ml,蛴螬组灌胃蛴螬提取物1 g/ml,空白组、模型组灌胃等体积生理盐水,1次/d.除空白组外,其余各组兔以氩激光照射视网膜静脉主干的方法造模,分别于造模后即时及造模后1、14、28 d行眼底照相和荧光素眼底血管造影(fluorescein fundus angiograph, FFA)检查,分别于1、3、7、14、28 d,采用免疫组化染色观察兔视神经CD40表达.结果 FFA结果显示,模型组造模后1 d静脉不充盈, 14 d仍有部分静脉不显影,28 d无血管再通迹象.血栓通组与蛴螬组造模后1 d部分静脉不充盈,14 d静脉充盈时间较模型组明显缩短,28 d 静脉充盈时间恢复到正常水平.免疫组化结果显示,模型组 1 d小胶质细胞明显增多,3 d小胶质细胞数量达最高峰,7、14、28 d小胶质细胞数量较前依次减少.造模后3、7、14、28 d,与模型组比较,血栓通组和蛴螬组大鼠CD40 [3 d:(8 908.91±96.30)、(6 099.92±273.44)比(10 436.4±1 306.8);7 d:(5 982.06±483.37)、(2 957.36±424.19)比(8 798.12±444.39);14 d:(3 225.36± 468.88)、(342.04±64.56)比(5 356.74±439.16);28 d:(756.97±80.17)、(72.85±11.06)比(4 215.27± 361.00)]表达降低(P<0.05).结论 血栓通与蛴螬提取物可不同程度抑制视网膜中央静脉阻塞模型兔视神经小胶质细胞活化,而蛴螬提取物的抑制作用更明显.
Objective To study the effect and mechanism of the extract from grub on the CD40 of microglia cells in rabbit model of central retinal vein occlusion. Methods The 60 color rabbits were randomly divided into the blank group, model group, Xueshuantong group and sputum group. The Xueshuantong group was intragastrically administered with Xueshuantong tablets suspension 5 mg/ml, the sputum group was given gavage extract 1 g/ml, and the blank group and the model group were intragastrically administered with normal saline once daily. Except for the blank group, the other groups of rabbits were modeled by argon laser irradiation of the retinal vein trunk, and the fundus photography and FFA examination were performed immediately after modeling and at 1, 14 and 28 days after modeling, respectively, at 1, 3, 7, 14 and 28 day, the expression of CD40 in the optic nerve of rabbits was observed by immunohistochemical staining. Results The FFA results showed that the veins were not filled at 1 day after model establishment, and some veins were not developed at 14 days, and there was no evidence of revascularization at 28 days. Part of the venous in the thrombus group and the sputum group was not filled. The venous filling time was significantly shorter than that in the model group at 14 days, and the venous filling time returned to the normal level at 28 days. The results of immunohistochemistry showed that the number of microglia increased significantly at 1 d in model group. The number of microglia reached the highest peak at 3 d, and the number of microglia decreased at 7 d, 14 d and 28 d. Compared to the model group, at the 3, 7, 14, 28 d, the integrated optical density of CD40 (3 d: 8 908.91 ± 96.30, 6 099.92 ± 273.44 vs. 10 436.4 ± 1 306.8;7 d: 5 982.06 ± 483.37, 2 957.36 ± 424.19 vs. 8 798.12 ± 444.39;14 d:3 225.36 ± 468.88, 342.04 ± 64.56 vs. 5 356.74 ± 439.16;28 d: 756.97 ± 80.17, 72.85 ± 11.06 vs. 4 215.27 ± 361.00) in the Xueshuantong group and sputum group significantly decreased ( P<0.05). Conclusions Th
作者
董子奕
蒋鹏飞
彭清华
Dong Ziyi;Jiang Pengfei;Peng Qinghua(College of Traditional Chinese Medicine,Hunan University of Traditional Chinese Medicine,Changsha 410208,China;Department of Ophthalmology,Tianjin People's Hospital,Tianjin 300121,China;Department of Ophthalmology,First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China;Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha 410208,China)
出处
《国际中医中药杂志》
2019年第10期1101-1106,共6页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金(30772824、81574031)
中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018)
长沙市科技计划项目(K1501014-31、kcl704005)
中央财政支持地方高校建设项目(2018年)
国家中医药管理局中医眼科学重点学科建设项目(ZK1801YK015)。
