摘要
目的研究在人不同瘢痕组织及增生性瘢痕不同生长时期成纤维细胞中甲基化结合蛋白2(methyl CpG binding protein 2,MECP2)和组蛋白去乙酰化酶6(histone deacetylase 6,HDAC6)的表达及意义。方法收集2016年8月至2017年5月重庆医科大学附属第一医院烧伤整形科收治的118例瘢痕患者及33例上睑松弛患者的手术标本,包括正常皮肤33例(男12例,女性21例,年龄34~68岁);正常瘢痕32例(男19例,女13例,年龄8~68岁);瘢痕疙瘩35例(男11例,女24例,年龄11~62岁);增生性瘢痕51例(男22例,女29例,12~58岁)。其中增生性瘢痕根据生长时间分为4组:0~3个月组10例、4~6个月组11例、7~12个月组13例、>12个月组17例。采用免疫组织化学及蛋白质印迹法检测各组组织中MECP2及HDAC6的表达情况,实时定量聚合酶链反应技术检测各组组织中MECP2 mRNA和HDAC6 mRNA的表达,并比较各组之间的差异。实验所得数据用SPSS 20.0进行统计学分析,各组之间的差异采用单因素方差分析进行检验,2组间比较采用t检验,P<0.05为差异有统计学意义。结果免疫组织化学结果显示,MECP2在正常皮肤成纤维细胞中表达呈阴性或弱阳性,在正常瘢痕中表达呈弱阳性,在增生性瘢痕中表达呈阳性,在瘢痕疙瘩成纤维细胞核内大量表达。MECP2蛋白在正常皮肤(1326.4±572.3)、正常瘢痕(2341.4±816.2)、增生性瘢痕(3500.7±1407.6)、瘢痕疙瘩(4787.9±1514.3)中的表达逐渐增加(F=33.820,P=0.001);同样MECP2 mRNA在正常皮肤(0.82±0.43)、正常瘢痕(1.14±0.45)、增生性瘢痕(1.59±0.39)、瘢痕疙瘩(2.14±0.53)中的表达也是逐渐增加(F=23.400,P=0.001)。在正常皮肤(1332.5±746.7)、正常瘢痕(2307.7±1027.4)、增生性瘢痕(4107.4±1223.1)、瘢痕疙瘩(5155.9±1265.3)中HDAC6蛋白的表达差异有统计学意义(F=50.270,P=0.001);HDAC6 mRNA在正常皮肤(0.57±0.23)、正常瘢痕(1.03±0.35)、增生性瘢痕(1.47±0.31)、瘢痕疙瘩(1.87±0.45)中的表达差异有统计
Objective To investigate the expression and significance of fibroblasts methyl CpG binding protein 2(MECP2)and histone deacetylase 6(HDAC6)at different stages of scar tissues and hypertrophic scars.Methods From August 2016 to May 2017,118 cases of scar and 33 cases of upper eyelid lasia received from the Department of Plastic and Burn Surgery of the First Affiliated Hospital of Chongqing Medical University,Chongqing were collected,including 33 cases of normal skin(ages 34-68,12 cases of male and 21 cases of female),32 cases of normal scar(ages 8-68,19 cases of male and 13 cases of female),35 cases of keloid(ages 11-62,11 cases of male and 24 cases of female)and 51 cases of hypertrophic scar(ages 12-58,22 cases of male and 29 cases of female)without any treatment.The hypertrophic scar was divided into 4 groups according to the growth time:10 cases in the 0-3 month group,11 cases in the 4-6 month group,13 cases in the 7-12 month group,and 17 cases in the>12 month group.Immunohistochemistry and western blot were used to detect the expressions of MECP2 and HDAC6 in tissues of each group,and real-time quantitative polymerase chain reaction gene amplification fluorescence detection technology was used to detect the expressions of MECP2mRNA and HDAC6 mRNA,and the differences between each group were compared.SPSS20.0 was used for statistical analysis of the data obtained in the experiment,and one-way ANOVAwas used to test the differences between the groups.P<0.05 was considered statistically significant.Results MECP2 protein is gradually expressed in normal skin(1326.4±572.3),normal scar(2341.4±816.2),hypertrophic scar(3500.7±1407.6)and keloid(4787.9±1514.3),F=33.82,P=0.001.Similarly,the expression of MECP2mRNA in normal skin(0.82±0.43),normal scar(1v14±0.45),hypertrophic scar(1.59±0.39)and keloid(2.14±0.53)was also gradually increased,F=23.4,P=0.001.In normal skin(1332.5±746.7)and normal scar(2307.7±1027.4),hypertrophic scar(4107.4±1223.1)and keloid,the level of HDAC6 protein expression(5155.9±1265.3)were si
作者
黎丹
赵娟
杨娥
张恒术
Li Dan;Zhao Juan;Yang E;Zhang Hengshu(Department of Plastic and Burn Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing,400016,China)
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2019年第10期1019-1026,共8页
Chinese Journal of Plastic Surgery
