摘要
该文研究了不同温度(35,45,55℃)胁迫对金银花膜透性、活性氧代谢及有效物质积累的影响,为金银花采后加工过程中减少劣变发生、揭示褐变机制提供理论基础。选取三青期金银花为实验材料,采用恒温干燥的方法,研究金银花细胞膜透性(相对电导率、MDA含量)、活性氧代谢(SOD,POD,PPO,CAT酶活活性)及有效物质(绿原酸,木犀草苷,新绿原酸,隐绿原酸,3,5-二咖啡酰奎宁酸,3,4-二咖啡酰奎宁酸,4,5-二咖啡酰奎宁酸)积累规律;采用SPSS 17.0统计软件分析数据。结果显示35℃胁迫下金银花中MDA含量较干燥初始时提高151.14%,干燥至0.150 g时金银花SOD,POD,PPO及CAT酶活活性分别较同等质量45℃胁迫下高出29.73%,42.86%,105.02%,10.74%;不同温度下金银花相关有效物质含量高低顺序为:35℃金银花>45℃金银花>55℃金银花。不同温度胁迫显著影响着金银花膜透性、活性氧代谢酶活性及有效成分积累的变化,各项指标显示35℃胁迫下金银花生理及活性氧代谢酶活活性最高,绿原酸、木犀草苷等成分得到有效积累,为金银花的采后加工过程中解决褐变问题提供基础数据。
The study is aimed to explore the effects of stress at different temperatures(35,45,55℃)on membrane permeability,active oxygen metabolism and accumulation of effective substances in Lonicera japonica,and provide theoretical basis for reducing deterioration and revealing browning mechanism during postharvest processing of L.japonica.The cell membrane permeability(relative conductivity,MDA content),active oxygen metabolism(SOD,POD,PPO,CAT activity)and the accumulation of effective substances(chlorogenic acid,luteolin,neochlorogenic acid,cryptochlorogenic acid,3,5-dicaffeoylquinic acid,3,4-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid)of L.japonica were all studied by constant temperature drying method,and the results were analyzed by the SPSS 17.0 statistical software.The results showed that MDA content in L.japonica was increased by 151.14%at 35℃,SOD,POD,PPO and CAT activity were 29.73%,42.86%,105.02%and 10.74%higher than at 45℃,respectively.The order of effective substance content in L.japonica was 35℃>45℃>55℃.The changes of membrane permeability,activity of active oxygen metabolizing enzyme and accumulation of active components were significantly affected by different temperature stress.The indexes showed that physiological and active oxygen metabolizing enzyme activity of L.japonica was the highest under 35℃stress,chlorogenic acid and luteolin were effectively accumulated,which provides basic data for solving browning problem in the postharvest processing of L.japonica.
作者
周冰谦
卢恒
刘峰
王晓
耿岩玲
刘伟
张华倩
ZHOU Bing-qian;LU Heng;LIU Feng;WANG Xiao;GENG Yan-ling;LIU Wei;ZHANG Hua-qian(Qilu University of Techiwlogy(Shandong Academy of Sciences),Shandong Analysis and Test Center,Shandong Key Laboratory of Traditional Chinese Medicine Qiuilily Control Technology,Ji'nan 250014,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第18期3935-3941,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81473298)
国家“十三五”重大专项(2017YFC1702701,2017YFC1700703)
现代农业产业技术体系建设专项(CARS-21)
山东省重点研发计划项目(2018GNC110040)
关键词
金银花
温度胁迫
活性氧代谢
有效物质
Lonicera japonica
temperature stress
active oxygen metabolism
active components
