摘要
目的:以气相色谱法测量姜黄挥发油中月桂烯、2-壬酮、异龙脑、莪术醇的含量,拟建立其含量测定方法,为姜黄质量控制提供依据。方法:采用气相色谱法,Elite-WAX ETR(0.25μm×0.25 mm×30 m)柱;程序升温:初始温度40℃,以7℃/min升至130℃,再以10℃/min升至200℃,保持15 min;载气为高纯氮气(≥99.999%),载气流量2 mL/min,分流比为10∶1;检测器:FID,检测器温度280℃,进样口温度250℃。结果:四种物质的方法学考察均符合要求,准确测定了10个批次姜黄挥发油样品中月桂烯、2-壬酮、异龙脑、莪术醇的含量。结论:气相色谱法测定姜黄挥发油中月桂烯、2-壬酮、异龙脑、莪术醇的含量简便、快捷,可用于控制姜黄的质量。
Objective:To accurately measure the content of myrcene,2-nonanone,isoborneol and curcumol in volatile oil from Jianghuang(Curcumae Longae Rhizoma) by gas chromatography. Methods:Gas chromatography,Elite-WAX ETR(0.25 μm×0.25 mm×30 m) column;program temperature :the initial temperature of40 ℃,with 7 ℃/min rise to 130 ℃,then 10 ℃/min rise to 200 ℃ for 15 min;carrier gas was high purity nitrogen(≥99.999%),carrier gas flow rate was 2 mL/min,split ratio was 10∶1;detector:FID,detector temperature was 280 ℃,inlet temperature was 250 ℃. Results:The methodological investigations of the four substances all met the requirements. The content of myrcene,2-nonanone,isoborneol and curcumol in 10 batches of volatile oil samples from Jianghuang(Curcumae Longae Rhizoma) were accurately determined. Conclusions:The determination of myrcene,2-nonanone,isoborneol,and curcumol in volatile oil of Jianghuang(Curcumae Longae Rhizoma) by GC method is simple and rapid,and can be used to control the quality of Jianghuang(Curcumae Longae Rhizoma).
作者
王秀兰
洪宗超
王金波
孙婉瑾
段雪云
WANG Xiulan;HONG Zongchao;WANG Jinbo;SUN Wanjin;DUAN Xueyun(Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China;Hubei Province Academy of Traditional Chinese Medicine,Wuhan 430074,China)
出处
《山东中医药大学学报》
2019年第5期513-518,共6页
Journal of Shandong University of Traditional Chinese Medicine
基金
湖北省院所项目(鄂食药监办文〔2015〕28号)
关键词
姜黄
气相色谱法
含量测定
质量控制
月桂烯
2-壬酮
异龙脑
莪术醇
Jianghuang(Curcumae Longae Rhizoma)
gas chromatography
content determination
quality control
myrcene
2-nonanone
isoborneol
curcumol
