摘要
壳聚糖作为一种天然的阳离子聚合物,生物相容性好,是理想的微胶囊壁材材料之一;而鱼油是一种易氧化的材料,低温有利于鱼油加工,采用冻干技术可以缓解鱼油的氧化作用。实验以壳聚糖为壁材,金枪鱼油为芯材,采用冷冻干燥法制备鱼油微胶囊,并运用响应面分析法优化制备工艺参数。结果表明:以包埋率为评价指标,在单因素试验的基础上根据Box-Behnken中心组合试验设计原理,结合实际生产情况,最终确定最优的微胶囊制备工艺参数为壁材质量浓度2.1%、壁芯比1.9∶1、乳化剂Tween80和Span80比为2∶1、乳化剂添加量0.9%,在此条件下鱼油微胶囊理论包埋率为79.67%,实际平均值为79.45%,所制得微胶囊理化指标符合行业标准。研究结果不仅对冷冻干燥法制备鱼油微胶囊具有一定的实践参考意义,也为壳聚糖微胶囊的制备提供一定的实验理论基础。
As a natural cationic polymer,chitosan has good biocompatibility and is one of the ideal wall materials of microcapsules,while fish oil is oxidizable material.Low temperature is beneficial to the processing of fish oil.Freeze-drying technology can alleviate the oxidation of fish oil.Therefore in this experiment,microcapsules of fish oil are made by freeze-drying method,with chitosan as wall material and tuna oil as core material,and response surface analysis is used to optimize the preparation process parameters.Results show that,with the encapsulation efficiency as the evaluation index,according to the design principle of Box-Behnken center combined test based on single factor test,and in combination with the actual production situation,the optimal preparation process parameters of microcapsules are confirmed to be 2.1%of wall material concentration,1.9∶1 of wall-core ratio,2∶1 of the ratio of emulsifiers Tween80 to Span80,and 0.9%of emulsifier content.Under such conditions,the theoretical value of encapsulation efficiency is 79.67%,while the actual average value is 79.45%.The physical and chemical indexes of microcapsules made are up to industrial standards.The results of this study not only can provide a practical reference for preparation of microcapsules of fish oil by freeze-drying method,but also provide an experimental theoretical basis for the preparation of chitosan microcapsules.
作者
李思敏
彭茜
戴志远
叶繁
LI Simin;PENG Xi;DAI Zhiyuan;YE Fan(Institute of Sea Food,Zhejiang Gongshang University,Hangzhou 310012,Zhejiang,China;State Key Laboratory of Aquatic Products Processing of Zhejiang Province,Hangzhou 310012,Zhejiang,China)
出处
《渔业现代化》
CSCD
2019年第5期82-88,共7页
Fishery Modernization
基金
国家重点研发计划课题“海洋活性脂质的高效制备技术及产品开发”(2018YFC0311204)
关键词
微胶囊
冷冻干燥法
响应面法
壳聚糖
鱼油
microcapsules
freeze-drying method
response surface method
chitosan
fish oil
