摘要
目的:探究环状RNA(circRNA)hsa_circ_0008797在胃癌顺铂耐药细胞及敏感细胞中的表达差异性;并通过敲低和调高hsa_circ_0008797在细胞中的表达,检测胃癌细胞顺铂耐药性的变化。方法提取胃癌顺铂敏感细胞株AGS、BGC-823、SGC7901及胃癌顺铂耐药细胞株SGC7901/DDP中的RNA,采用实时荧光半定量PCR(RT-PCR)检测各胃癌细胞株中hsa_circ_0008797的相对表达量。通过shRNA干扰SGC7901细胞中hsa_circ_0008797的表达,同时对SGC7901/DDP细胞中hsa_circ_0008797进行过表达;采用CCK-8法、流式细胞技术检测各细胞的顺铂耐药性变化。结果hsa_circ_0008797在胃癌顺铂敏感细胞株AGS、BGC-823、SGC7901中的表达量相对较高,而在胃癌顺铂耐药细胞株SGC7901/DDP中的表达量相对较低(P<0.05)。过表达hsa_circ_000879后,胃癌细胞SGC7901/DDP对顺铂的耐药性下降(P<0.05);敲低hsa_circ_000879的表达后,胃癌细胞SGC7901对顺铂的耐药性增加(P<0.05)。结论hsa_circ_000879可逆转胃癌细胞对顺铂的耐药性,可能在胃癌顺铂耐药性产生过程中发挥着一定作用。
Objective To investigate the differential expression of circular RNA hsa_circ_0008797 in cisplatin-resistant and sensitive cells of gastric cancer,and to detect the effect of downregulate and upregulatethe expression of circRNA on cisplatin resistance of gastric cancer cells.Methods The RNAs,which from gastric cancer cisplatin-sensitive cell lines AGS,BGC-823,SGC7901 and gastric cancer cisplatin-resistant cell line SGC7901/DDP were extracted.The expression level of hsa_circ_0008797 in each gastric cancer cell line was detected by real-time fluo-rescent semi-quantitative PCR(RT-PCR).The expression of hsa_circ_0008797 in SGC7901 cells was silenced by shRNA,and hsa_circ_0008797 was overexpressed in SGC7901/DDP cells.CCK-8 method and flow cytometry were used to detect the changes of cisplatin resistance in each cell line.Results The expression of hsa_circ_0008797 was higher in cisplatin-sensitive cell lines AGS,BGC-823 and SGC7901,but lower in cisplatin-resistant cell line SGC7901/DDP(P<0.05).After overexpression of hsa_circ_0008797,the resistance of gastric cancer cells SGC7901/DDP to cisplatin decreased((P<0.05).After knocking down the expression of hsa_circ_0008797,the resistance of gastric cancer cell SGC7901 to cisplatin was significantly increased(P<0.05).Conclusion hsa_circ_0008797 can reverse the resistance of gastric cancer cells to cisplatin,which may play a role in the process of cisplatin resistance in gastric cancer.
作者
刘文博
高敏
刘婉婷
顾康生
Liu Wenbo;Gao Min;Liu Wanting(Dept of Oncology,The First Affiliated Hospital of Anhui Medical University,Hefei230022)
出处
《安徽医科大学学报》
CAS
北大核心
2019年第11期1702-1707,共6页
Acta Universitatis Medicinalis Anhui
基金
安徽省对外科技合作计划项目(编号:1604b0602027)
安徽省自然科学基金(编号:1808085MH306)
关键词
胃癌
circRNA
顺铂
化疗耐药
gastric cancer
circRNA
cisplatin
chemotherapy resistance
