摘要
本研究选用DPPH自由基清除率、ABTS自由基清除率、铁离子还原力和总还原力四种体外检测体系,发现刺玫果黄酮和皂苷成分具有较强的抗氧化能力,且呈一定的剂量依赖效应。各浓度的样品对ABTS自由基清除作用效果均优于Vc。其中30%乙醇洗脱的刺玫果皂苷成分,在200μg/mL时的清除率已达到98.78%;800μg/mL时,50%乙醇洗脱的刺玫果皂苷的DPPH自由基清除率达到了91.88%,非常接近Vc;黄酮和皂苷成分对铁离子的还原力均高于1.50,接近Vc的1.72;30%乙醇洗脱的皂苷成分,总还原力为2.07接近Vc的2.28;通过衰老模型下的HaCaT细胞修复实验,发现刺玫果的黄酮和皂苷成分具有较好的抗衰老活性,可以修复受损伤的细胞形态,并提高其存活率,且呈现出一定的剂量依赖性。800μg/mL时,四个样品(30%乙醇洗脱的黄酮、50%乙醇洗脱的黄酮、30%乙醇洗脱的皂苷、50%乙醇洗脱的皂苷)的修复率分别为为371.94%、394.39%、392.35%、399.83%,均接近Vc的404.25%。
In this study,the flavonoids and saponins from Rosa davurica Pall were found to have strong antioxidant capacity and exhibit dose-dependence to some extent in DPPH free radical scavenging assay,ABTS free radical scavenging assay,ferric-reducing antioxidant power(FRAP)assay,and total reducing power assays.The test samples at all the examined concentrations exhibited higher ABTS free radical scavenging rate than Vc,among which the saponin components in the 30%ethanol-eluted fraction(at 200μg/mL)had an ABTS scavenging rate of 98.78%;At 800μg/mL,the saponins in the 50%ethanol-eluted fraction had a DPPH free radical scavenging rate of 91.88%(which was very close to that for Vc).In FRAP assay,the reducing power of the flavonoids and saponins was higher than 1.50 which was very close to that for Vc(1.72).The total reducing power of the saponin components in the 30%ethanol-eluted fraction was 2.07(which was close to that for Vc 2.28).In the aging model based on HaCaT cell repair,the flavonoids and saponins from Rosa davurica Pall were found having good anti-aging activity,and could repair the morphology of damaged cells,increased their survival rate,and showed dose-dependence to some extent.At 800μg/mL,the repair rate of the four test samples(the 30%ethanol-eluted flavonoids,50%ethanol-eluted flavonoids,30%ethanol-eluted saponins,and 50%ethanol-eluted saponins)were 371.94%,394.39%,392.35%,and 399.83%,respectively,which were all close to that for Vc(404.25%).
作者
宋德幸
姜建国
SONG De-xing;JIANG Jian-guo(College of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)
出处
《现代食品科技》
EI
CAS
北大核心
2019年第10期110-115,共6页
Modern Food Science and Technology
基金
广州市科技计划项目(201604020150)
关键词
刺玫果
黄酮
皂苷
抗氧化
抗衰老
Rosa davurica Pall
flavonoids
saponins
antioxidant
anti-aging
