摘要
雨生红球藻是一种重要的资源微藻,产物虾青素具有超强抗氧化活性,因而广泛被应用于医药和食品等领域。为雨生红球藻实现高产虾青素,研究了3种胁迫条件,光照强度(5 000、7 000、9 000 lx)、硝酸钠浓度(0、0.13、0.25 g/L)和氯化钠浓度(0、2.5、5.0 g/L)对雨生红球藻的虾青素含量的影响,利用响应面法对胁迫条件进行优化。3因素对产生虾青素含量的影响顺序为:氯化钠浓度>光照强度>硝酸钠浓度。优化得到的最佳提取条件为光照强度9 000 lx、硝酸钠浓度0 g/L、氯化钠浓度2.5 g/L,在此条件下虾青素含量可达6.8 mg/L,与预测值相近,表明模型具有较高可靠性。
Haematococcus pluvialis is an important resource microalgae.The product astaxanthin has excellent antioxidant activity and is widely used in medicine and food.In order to study the high-yield astaxanthin of Haematococcus pluvialis,three stress conditions(5 000,7 000,9 000 lx),sodium nitrate concentrations(0,0.13,0.25 g/L)and chlorination were studied.The effects of sodium concentrations(0,2.5,5.0 g/L)on astaxanthin content of Haematococcus pluvialis were optimized by response surface methodology.The order of influence of the three factors on the production of astaxanthin content was:sodium chloride concentration>light intensity>sodium nitrate concentration.The optimal extraction conditions were:light intensity 9 000 lx,sodium nitrate concentration 0 g/L,sodium chloride concentration 2.5 g/L,under which the astaxanthin content could reach 6.8 mg/L,and it was similar to the predicted value.The results indicated that the model has high reliability.
作者
李嘉仪
窦勇
邵蓬
高金伟
贾旭颖
张文慧
王祎哲
马婷
周文礼
LI Jia-yi;DOU Yong;SHAO Peng;GAO Jin-wei;JIA Xu-ying;ZHANG Wen-hui;WANG Yi-zhe;MA Ting;ZHOU Wen-li(Tianjin Fisheries Resources and Environment Research Office,Tianjin Agricultural University,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2019年第3期57-61,共5页
Journal of Tianjin Agricultural University
基金
天津市水产产业技术体系创新团队项目（ITTFRS2017005）
天津市科技重大专项与工程项目（15ZXBFNC00120）
卫星海洋环境动力学国家重点实验室开放基金项目（SOED1419）
农业部南海渔业资源开发利用重点实验室开放基金项目（FREU2015-04）
天津市水产生态及养殖重点实验室开放基金项目（TJAE201805）
关键词
雨生红球藻
虾青素
光照强度
响应面
Haematococcus pluvialis
astaxanthin
light intensity
response surface
