摘要
通过蔗糖、H3BO3、CaCl2单因子及L9(33)正交试验,筛选毛棉杜鹃花粉萌发的最佳培养基,并比较了TTC、醋酸洋红和I2-KI染色法对快速测定毛棉杜鹃花粉活力的影响。结果表明:毛棉杜鹃花粉在蔗糖300 g·L^-1、H3BO3 200mg·L^-1、CaCl2 500mg·L^-1的培养基上,萌发率最高;醋酸洋红染色法是简单快速测定毛棉杜鹃花粉活力的理想方法。
This experiment was conducted with the pollen grains of R.moulmainense Hook.f.from Wutong mountain in Shenzhen.The effects of sucrose,H3BO3 and CaCl2 single factor and orthogonal test L9(33)on R.moulmainense Hook.f.pollen germination were investigated.The TTC,I2-KI and aceto carmine were compared to find the fastest determination method of R.moulmainense Hook.f..The results indicated that the optimal germination medium of pollen was sucrose 200g·L^-1,H3BO3100mg·L^-1 and CaCl2 300mg·L^-1 and aceto carmine is the optimum and rapid staining method to determine pollen vitality.
作者
白宇清
王定跃
谢利娟
Bai Yuqing(Administrative Office of Wutong Mountain National Park,Shenzhen 518004,China)
出处
《安徽农学通报》
2019年第18期13-15,共3页
Anhui Agricultural Science Bulletin
基金
深圳市城市管理局科研基金(SZ201201)
