摘要
以培养好的细菌、霉菌、酵母菌、放线菌为实验材料,在CTAB法基础上配制成了含有dissociation buffer、purification buffer、DNA wash buffer和elution buffer 4种缓冲液的试剂盒,并增加了DNA收集柱.采用该试剂盒分别提取了细菌、霉菌、酵母菌、放线菌的DNA.分别采用感官、核酸检测仪Agilent 2100、NanoDropRND-1000和琼脂糖凝胶方法评定了提取到的DNA质量.试验结果表明:利用该试剂盒可以获得4种微生物的高浓度和高纯度DNA,其操作简单,费用低廉.
Bacteria,mold fungus,yeast and actinomycetes are experimental materials.We made of DNA extraction kit with four buffers solution(dissociation buffer,purification buffer,DNA wash buffer and elution buffer)on base of the CTAB method.DNA of bacteria,mold fungus,yeast and actinomycetes were extracted.Then,the four kinds of microbial DNA were detected by sense organ,Agilent 2100,NanoDrop ND-1000 and agarose gel electrophoresis.The results showed that the DNA extraction kit with low prices can gain high concentration and high purity DNA and the operation process is simple.
作者
张杰
李研
杨琳
李新欣
赵新
候珺淇
代振玉
ZHANG Jie;LI Yan;YANG Lin;LI Xinxin;ZHAO Xin;HOU Junqi;DAI Zhenyu(College of Life Science and Agronomy,Zhoukou Normal University,Zhoukou 466001,China)
出处
《周口师范学院学报》
CAS
2019年第5期74-77,共4页
Journal of Zhoukou Normal University
基金
周口师范学院高层次人才科研启动经费研究项目
关键词
细菌
霉菌
酵母菌
放线菌
DNA
bacteria
mold fungus
yeast
actinomycetes
DNA
