摘要
基于阿米卡星对纳米金-罗丹明B体系的荧光增强,建立了一种快速、灵敏测定硫酸阿米卡星的新方法。通过对反应时间、纳米金与RhB配比、温度等实验条件的优化,在优化的实验条件下,方法的线性范围为7.80×10^-6~1.25×10^-4 mol/L,检出限为2.2×10^-6 mol/L,该方法用于检测硫酸阿米卡星针剂中的阿米卡星的含量,效果良好。
Based on the fluorescence enhancement of amikacin to gold nanoparticles-rhodamine B system,a new method for rapid and sensitive determination of amikacin was established.The experiment conditions such as the reaction time,ratio of gold nanoparticles to rhodamine B and the reaction temperature were optimized.Under the optimized experimental conditions,the linear range of the method is 7.80×10^-6~1.25×10^-4 mol/L,and the detection limit is 2.2×10^-6 mol/L.The method was used to detect amikacin in Amikacin sulfate injection and the results were similar to the notated value.
作者
王睿
田锐
刘航航
张美琪
何思媛
WANG RUI;TIAN RUI;LIU Hang-hang;ZHANG Mei-qi;HE Si-yuan(School Chemistry and Chemical Engineering,Yan′an University/Yan′an Key Laboratory of Analytial Technology and Testing,Yan′an 716000,China)
出处
《延安大学学报（自然科学版）》
2019年第3期45-48,共4页
Journal of Yan'an University：Natural Science Edition
基金
延安大学博士科研启动项目(YDBK2017-05)
延安大学大学生创新创业训练计划项目(D2018022)
陕西省大学生创新创业训练计划项目(S201910719042)
关键词
纳米金
罗丹明B
荧光增强
阿米卡星
gold nanoparticles
rhodamine B
fluorescence enhancement
amikacin
