摘要
目的观察养阴柔肝、化瘀息风法对自发性高血压大鼠(SHR)血压及炎症因子的影响。方法将144只8周龄SPF级雄性SHR大鼠随机分为模型组、MRS2578组、复方七芍降压片组、厄贝沙坦组、MRS2578+复方七芍降压片组、MRS2578+厄贝沙坦组,每组24只;另选择24只健康、SPF级雄性WKY大鼠作为对照组。模型组和对照组大鼠给予蒸馏水灌胃,其他各组大鼠给予相应药物干预,其中复方七芍降压片流浸膏剂量为0.65 mL,厄贝沙坦剂量为3.63 mg,MRS2578按3 mg/kg腹腔注射(隔日1次)。各组灌胃均1次/d,连续8周。记录各组实验前及实验开始后每周最后一天14:00—17:00每只大鼠的尾动脉血压,采用ELISA法检测各组大鼠实验第1,4,8周最后一天血清中白细胞介素-6(IL-6)、IL-1β、肿瘤坏死因子-α(TNF-α)水平。结果对照组大鼠血压及血清中IL-6、IL-1β、TNF-α水平在整个实验阶段均比较平稳;模型组大鼠血压及血清中IL-6、IL-1β、TNF-α水平自发性升高,均明显高于同时点对照组(P均<0.05);各药物干预组大鼠血压及血清中IL-6、IL-1β、TNF-α水平均呈下降趋势,第4周末开始均明显低于同时点模型组(P均<0.05),且MRS2578+复方七芍降压片组均明显低于MRS2578组和复方七芍降压片组(P均<0.05),MRS2578+厄贝沙坦组均明显低于厄贝沙坦组和MRS2578组(P均<0.05);复方七芍降压片组与厄贝沙坦组比较、MRS2578+复方七芍降压片组与MRS2578+厄贝沙坦组比较各指标差异均无统计学意义(P均>0.05)。结论养阴柔肝、化瘀息风法可以有效降低SHR大鼠的血压及血清炎性因子水平,可直接从抑制炎症反应的源头上维持机体促炎-抗炎平衡,缓解高血压病情。
Objective It is to observe the effects of Yangyin Rougan and Huayu Xifeng on blood pressure and inflammatory factors in the rats with spontaneously hypertension(SH).Methods A total of 144 8-week-old SPF male SH rats were randomly divided into model group,MRS2578 group,compound Qishao Jian gya tablet group,irbesartan group,MRS2578+compound Qishao Jiangya tablet group,MRS2578+irbesartan groups,24 rats in each group.Another 24 healthy SPF male WKY rats were selected as the control group.The rats in the model group and the control group were given distilled water by gavage,and the rats in the other groups were treated with the corresponding drug.The dose of compound Qishao Jiangya tablets was 0.65 mL,the dose of irbesartan was 3.63 mg,and the dose of MRS2578 was 3 mg/kg for intraperitoneal injection(once every other day).The intragastric administration was performed once a day for 8 weeks.The blood pressure of the tail artery of each rat before and after the start of the experiment was recorded from 14:00 to 17:00 on the last day of each week.The levels of serum interleukin-6(IL-6),IL-1β,and tumor necrosis factor-α(TNF-α)on the last day of the first,fourth and eighth week of the experiment were detected by ELISA.Results The blood pressure and serum levels of IL-6,IL-1βand TNF-αin the control group were stable throughout the experimental period.The blood pressure and serum levels of IL-6,IL-1βand TNF-αin the model group were spontaneously increased,and they were significantly higher than that of the control group at the same time(P<0.05).The blood pressure and serum levels of IL-6,IL-1βand TNF-αin the drug intervention group showed a downward trend,they were significantly lower than those in the model group at the same time point from the end of the forth week(P<0.05),and these indexes in the MRS2578+compound Qishao Jiangya tablets group were significantly lower than those in the MRS2578 group and the compound Qishao Jiangya tablet group(P<0.05),MRS2578+irbesartan group was significantly lower than the irbesa
作者
王顺民
唐路军
曾勇
陆朵梅
周骞
黄露
雍苏南
谭元生
WANG Shunmin;TANG Lujun;ZENG Yong;LU Duomei;ZHOU Qian;HUANG Lu;YONG Sunan;TAN Yuansheng(The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,Hunan,China;Hunan University of Traditional Chinese Medicine,Changsha 410208,Hunan,China)
出处
《现代中西医结合杂志》
CAS
2019年第28期3091-3095,3118,共6页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
湖南省中医药科研计划重点项目(201753)
国家自然科学基金面上项目(81473616)
国家科学基金青年基金项目(81804077)
湖南省自然科学基金(青年基金)(2019JJ50468)
关键词
自发性高血压大鼠
养阴柔肝、化瘀息风法
原发性高血压
炎症因子
spontaneously hypertensive rats
therapy for nourishing yin and softening liver,removing blood stasis
essential hypertension
inflammatory factors
