摘要
目的将黄芪的药理活性与纳米材料的独特性能相结合,通过纳米材料提高黄芪的药理活性。方法以黄芪提取物为还原剂,使用生物还原法制备纳米银颗粒,分别通过动态光散射及抑菌杀菌试验,观察不同制备方法下纳米银颗粒表征、稳定性及抑菌杀菌能力。结果黄芪提取物制备的纳米银颗粒粒径为40.3nm,较空白纳米银颗粒小。伴随加速实验时间延长,黄芪纳米银及空白纳米银颗粒大小均呈现增大趋势。35d后空白纳米银颗粒粒径为增加为392.7nm,而黄芪提取物制备纳米银颗粒粒径为64.2nm,仍保持在100nm以下,增幅小于空白对照组。黄芪提取物有助于增加纳米银颗粒稳定性,避免纳米银颗粒过度聚集。黄芪提取物可显著提高纳米银的抗菌能力,大肠埃希菌的最低抑菌浓度为50mg/L,比白色纳米银颗粒大10倍以上。金黄色葡萄球菌的最低抑菌浓度仅为75mg/L,与空白纳米银颗粒相比抗菌活性增加了20倍,对于上述两种细菌最小杀菌浓度分别增加4倍和13倍。结论黄芪不仅可以提高纳米银颗粒的稳定性,还可以增强纳米银颗粒的抗菌活性。
Objective To combine the pharmacological activity of Astragalus membranaceus with the unique properties of nanomaterials,and to improve the pharmacological activity of Astragalus by nanomaterials.Methods Astragalus membranaceus extract was used as reducing agent to prepare nano-silver particles by biological reduction method.The dynamic yellow light scattering and antibacterial bactericidal tests were used to observe the characterization,stability and antibacterial bactericidal ability of nano-silver particles under different preparation methods.Results The particle size of the nano-silver particles prepared by the extract of Astragalus membranaceus was 40.3 nm,which was smaller than that of the blank nano-silver particles(84.2 nm).With the prolonged acceleration time,the size of Astragalus nano-silver and blank nano-silver particles increased.After 35 days,the particle size of the blank nano-silver particles increased to 392.7 nm,while the particle size of the nano-silver particles prepared by the extract of Astragalus membranaceus was 64.2 nm,which remained below 100 nm,which was smaller than that of the blank control group.Astragalus extract helps to increase the stability of the nano-silver particles and avoid excessive aggregation of the nano-silver particles.Astragalus membranaceus extract can significantly improve the antibacterial ability of nano-silver.The minimum inhibitory concentration of Escherichia coli is 50 mg/L,which is more than 10 times larger than white nano-silver particles.The minimum inhibitory concentration of Staphylococcus aureus was only 75 mg/L,and the antibacterial activity was increased by 20 times compared with the blank nanosilver particles.The minimum bactericidal concentration of the above two bacteria was increased by 4 times and 13 times,respectively. Conclusion Astragalus can not only improve the stability of nano-silver particles,but also enhance the antibacterial activity of nano-silver particles.
作者
毛迪
杜宇
赵婷婷
张立辰
刘志军
MAO Di;DU Yu;ZHAO Tingting;ZHANG Lichen;LIU Zhijun(Department of Clinical Medicine,Weifang Medical University,Weifang 261053,China;Department of Medical Microbiology,School of Clinical Medicine,Weifang Medical University)
出处
《潍坊医学院学报》
2019年第4期247-250,共4页
Acta Academiae Medicinae Weifang
基金
国家自然科学基金资助课题(项目编号:81471048)
山东省自然科学基金资助课题(项目编号:ZR2012HL42)
山东省高等学校科技计划项目资助课题(项目编号:J12LK01)
潍坊医学院博士启动基金(项目编号:2017BSQD01)
潍坊医学院大学生科技创新基金项目资助课题(项目编号:KX2017031)
关键词
纳米银
黄芪
抗菌
Nano-silver particles
Astragalus
Antibacterial
