摘要
目的探讨气道上皮人钙激活氯离子通道1(hCLCA1)表达水平与肺内源性急性呼吸窘迫综合征(ARDSp)患者肺部炎症水平、临床预后的相关性。方法入选2017年1月至2018年6月空军军医大学西京医院住院或门诊的肺病患者,按照疾病分为3组:ARDSp组(26例)、社区获得性肺炎(CAP)组(26例)及正常对照组(28例)。检测各组患者支气管肺泡灌洗液(BALF)中肿瘤坏死因子α(TNF-α)、hCLCA1水平及中性粒细胞百分比。其中ARDSp组选择治疗前及治疗7 d后2个时间点。结果与正常对照组比较,ARDSp组、CAP组BALF中hCLCA1、TNF-α、中性粒细胞百分比均显著升高(P值均<0.01),ARDSp组BALF中hCLCA1、TNF-α、中性粒细胞百分比高于CAP组(P<0.05或P<0.01)。将ARDSp患者分为生存组(14例)和死亡组(12例),治疗前死亡组BALF中hCLCA1、TNF-α水平均高于生存组(P值均<0.05);与治疗前比较,治疗7 d后ARDSp死亡组BALF中hCLCA1、TNF-α、中性粒细胞百分比差异无统计学意义,生存组则表现为下降(P值均<0.05)。直线相关分析显示,ARDSp患者BALF中hCLCA1与TNF-α呈正相关(r=0.823,P<0.05),中性粒细胞百分比与TNF-α呈正相关(r=0.417,P<0.05),同时与hCLCA1也呈正相关(r=0.430,P<0.05)。结论ARDSp患者BALF中hCLCA1水平显著高于CAP及正常肺叶患者,ARDSp死亡组BALF中hCLCA1水平高于生存组。ARDSp患者BALF中hCLCA1与TNF-α及中性粒细胞百分比呈正相关。推测hCLCA1与ARDSp的肺组织炎症水平及预后可能存在相关性。
Objective To explore the correlation of human calcium-activated chloride channel 1(hCLCA1)expression in airway epithelium with the pulmonary inflammation level and clinical prognosis of patients with pulmonary acute respiratory distress syndrome(ARDSp).Methods The patients with pulmonary diseases in Xijing Hospital,Air Force Military Medical University from January 2017 to June 2018 were divided into three groups according to diseases:ARDSp group(26 cases),community-acquired pneumonia(CAP)group(26 cases)and normal control group(28 cases).Tumor necrosis factor-α(TNF-α),hCLCA1 and neutrophil percentage in bronchoalveolar lavage fluid(BALF)were measured in each group.The above indexes of ARDSp group were detected before treatment and after treatment for seven days.Results Compared with normal control group,hCLCA1,TNF-αand neutrophil percentage in BALF of ARDSp group and CAP group increased significantly(all P<0.01).hCLCA1,TNF-αand neutrophil percentage in BALF of ARDSp group was higher than those of CAP group(P<0.05 or P<0.01).ARDSp patients were divided into survival group(14 cases)and death group(12 cases).Before treatment,the levels of hCLCA1 and TNF-αin BALF of death group were higher than those of survival group(all P<0.05).There was no significant difference in hCLCA1,TNF-αand neutrophil percentage in BALF of ARDSp death group between before treatment and after treatment for seven days,while the above indexes in survival group decreased after treatment for seven days compared with before treatment(all P<0.05).Linear correlation analysis showed that hCLCA1 was positively correlated with TNF-α(r=0.823,P<0.05),and neutrophil percentage was positively correlated with TNF-α(r=0.417,P<0.05)and hCLCA1(r=0.430,P<0.05)in BALF of ARDSp patients.Conclusions The level of hCLCA1 in BALF of patients with ARDSp is significantly higher than that of patients with CAP and normal pulmonary lobe.The level of hCLCA1 in BALF of death group is higher than that of survival group in ARDSp patients.hCLCA1 is positively correlat
作者
张天相
梁家宁
杨揆
郑龙
陈伟
张艳
宋立强
Zhang Tianxiang;Liang Jianing;Yang Kui;Zheng Long;Chen Wei;Zhang Yan;Song Liqiang(Department of Respiratory and Critical Care Medicine,Xijing Hospital,Air Force Military Medical University,Xi′an 710032,China)
出处
《国际呼吸杂志》
2019年第17期1308-1312,共5页
International Journal of Respiration
基金
国家自然科学基金面上项目(81570072)
陕西省社会发展科技攻关项目(2016SF-096)。
关键词
肺内源性急性呼吸窘迫综合征
社区获得性肺炎
人钙激活氯离子通道1
肿瘤坏死因子Α
中性粒细胞
Pulmonary endogenous acute respiratory distress syndrome
Community-acquired pneumonia
Human calcium-activated chloride channel 1
Tumor necrosis factor-α
Neutrophil
