摘要
目的通过Meta分析评估TLR4基因rs 1927914位点的基因多态性与缺血性脑卒中的易感性,旨在为缺血性脑卒中发病遗传易感性提供循证医学依据。方法检索收集2001年1月至2017年2月发表的有关TLR4基因多态性与缺血性脑卒中易感性的病例对照和队列研究文献。通过Newcastle-Ottawa量表对纳入的文献进行质量评价。应用Stata12.0软件对已纳入的文献数据进行Meta分析确定TLR4rs 1927914位点是否与缺血性脑卒中易感性相关,并通过敏感性分析判断Meta分析的稳定性,进行异质性检验以判断各研究间的异质性,亚组分析获取异质性来源。结果共13篇文献符合纳入标准,包括缺血性脑卒中患者4 334例和对照组7 745例,文献质量等级为中、高质量。TLR4 rs 1927914的T等位基因和缺血性脑卒中的患病风险关联性Meta分析结果显示,显性模型:OR=1.384,95%CI 1.062~1.804,P=0.016,共显性模型:OR=1.232,95%CI 1.002~1.515,P=0.048,隐性模型:OR=1.246,95%CI 0.912~1.703,P=0.168。将不同人群进行亚组分析发现在欧美人口中T等位基因和缺血性脑卒中的患病风险具有显著关联性(显性模型:OR=1.201,95%CI 1.080~1.322,P=0.021;共显性模型:OR=1.248,95%CI 1.002~1.494,P=0.043),但在亚洲人口中TLR4基因的rs1927914位点与缺血性脑卒中的关联性未见显著差异(显性模型:OR=0.667,95%CI 0.547~0.787,P=0.608;隐性模型:OR=1.152,95%CI 1.029~1.275,P=0.061;共显性模型:OR=0.725,95%CI 0.546~0.97,P=0.153)。结论TLR4基因rs 1927914位点上的T等位基因与缺血性脑卒中具有相关性,主要体现在欧美人群中,亚洲人群没有明显相关性。
Objective In this study,the rs1927914 locus polymorphism of TLR4 gene and susceptibility to ischemic stroke were evaluated by Meta-analysis to provide evidence-based medicine for genetic predisposition of ischemic stroke.Methods Searched for a cohort study and case-control literature on ischemic stroke from January 2001 to February 2017.To determine whether the TLR4 rs 1927914 locus was associated with ischemic stroke susceptibility by allele comparison and genotype-to-genotype comparisons.Newcastle-Ottawa was used for quality evaluation.Stata 12.0 software was used to perform Meta-analysis,heterogeneity and subgroup analysis.Results A total of 13 TLR4 rs 1927914 loci were associated with ischemic stroke susceptibility involving 4334 patients with ischemic stroke and 7745 controls.All the original studies were mid and high quality.Meta-analysis showed that TLR4 rs 1927914 dominant model(OR=1.384,95%CI 1.062-1.804,0.016),the co-dominant fixed effect model(OR=1.525,95%CI 1.416-1.930)and recessive model(OR=1.246,95%CI 0.912-1.703,P=0.168).Subgroup analysis of different populations revealed a significant association between T allele and the risk of ischemic stroke(dominant model:OR=1.201,95%CI 1.080-1.322,P=0.021;co-dominant model:OR=1.248,95%CI 1.002-1.494,P=0.043),but the rs1927914 locus of TLR4 gene was associated with the risk of ischemic stroke in Asian populations There was no significant correlation between ischemic stroke(dominant model:OR=0.667,95%CI 0.547-0.787,P=0.608;recessive model:OR=1.152,95%CI 1.029-1.275,P=0.061;co-dominant model:OR=0.725,95%CI 0.546-0.97,P=0.153).Conclusion The T allele at the rs 1927914 locus of the TLR4 gene is indeed a risk factor for ischemic stroke.Subgroups analysis show that when the T allele in Europe and America is high,ischemic stroke is more likely to occur.However,there is no effective evidence for the conclusion of the Asian population.
作者
刘远兴
王士中
刘险辉
谢运泉
易宏淦
LIU Yuanxing;WANG Shizhong;LIU Xianhui;XIE Yunquan;YI Honggan(Meizhou People′s Hospital,Meizhou,Guangdong 514031,China)
出处
《国际检验医学杂志》
CAS
2019年第18期2200-2204,共5页
International Journal of Laboratory Medicine
基金
广东省医学科学技术研究基金(B2017102)
梅州市科技计划项目(2017B035)
