摘要
为探究“蚓触圈”黏液对还田后玉米秸秆分解的作用,将玉米秸秆与烧灼过的石英砂混合,模拟还田后玉米秸秆分解过程。分别向该混合物中添加赤子爱胜蚓黏液、葡萄糖和谷氨酸。黏液总糖质量浓度为1.3 mg/mL,粗蛋白质量浓度为3.4 mg/mL。整个试验期间,黏液组碳氮矿化速率始终高于对照组,表明蚯蚓黏液对碳氮矿化具有促进作用,黏液的促进作用出现时间迟于葡萄糖和谷氨酸。对于细菌群落,黏液组ACE、Chao和Shannon-Wiener指数分别为630.24、640.08和4.90,对照组相应值分别为240.70、215.75和3.41。对于真菌群落,黏液组ACE、Chao和Shannon-Wiener指数分别为171.43、165.80和2.42,对照组相应值分别为71.56、70.58和0.50。表明黏液组微生物丰度和多样性均高于对照组。该研究结果表明,“蚓触圈”黏液对还田后玉米秸秆分解起着重要作用。
Returning crop residues to soil is regarded as a beneficial agricultural management practice.The decomposition of the returned organic residues is a research concern,and the soil fauna plays an important role during the degradation process.Earthworms are generally regarded as a major and beneficial component of soil fauna.In the present study,the decomposition process was simulated by mixing the maize stover with the ignited quartz sand.The mucus of the earthworm Eiseniafetida which was an important component of drilosphere,glucose or glutamate were added to the mixture.Besides,glucose and glutamate were easily utilizable substrate for the microorganism.The total carbohydrate and crude protein contents in the mucus were analyzed.The total carbohydrate content was 1.3 mg/mL,and the crude protein content was 3.4 mg/mL.SDS-PAGE showed that the protein in the mucus was mainly comprised of two specific protein molecules with molecular weight approximately 44.6 and 21.5 kDa.The carbon and nitrogen mineralization,enzyme activity and microbial community structure were investigated in detail.In the experiment,the carbon mineralization rates of Glc and Glu groups reached a peak value at around 10th day,while that of Muc group reached a maximum value at 20^th-30^th day.The maximum nitrogen mineralization rates in both Glc and Glu groups were in the 20th-30th day,while those in Muc group were in the 30th-40th day.The effect of the mucus was compared with that of glucose and glutamate.During the whole experiment process,the carbon mineralization rate of Muc group was always higher than that of the control group.After comparing with the control group,it could be observed that the mucus had stimulatory effect on carbon and nitrogen mineralization.The delay effect of the mucus was observed when it was compared to glucose and glutamate.The activities of protease and dehydrogenase in Muc group were higher than those in CK group.On the 10th day,the activities of carboxymethyl cellulase and urease in Muc group were 4 times and 1.5 t
作者
陈玉香
赵婷婷
姚月
徐立新
Chen Yuxiang;Zhao Tingting;Yao Yue;Xu Lixin(College of Biological and Agricultural Engineering,Jilin University,Changchun 130022,China;College of Life Science,Jilin University,Changchun 130022,China)
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2019年第15期234-240,共7页
Transactions of the Chinese Society of Agricultural Engineering
基金
国家自然科学基金项目(31872178)
关键词
秸秆
分解
蚯蚓黏液
微生物组成
straw
decomposition
earthworm mucus
microbial composition
