摘要
目的观察高钠饮食下大鼠血钠及血浆和心肌组织中血管活性物质内皮素-1(ET-1)、一氧化氮(NO)、内皮型一氧化氮合酶(eNOS)及eNOSmRNA昼夜节律变化,探讨高钠饮食引起血压昼夜节律异常的分子生物学机制。方法将64只SD大鼠随机分为2组,高钠组32只饮用含0.9%NaCl盐水,对照组32只饮去离子水。喂养8周后分别于2:00,8:00,14:00,20:00每组各随机处死8只,取血检测血钠及血浆ET-1、NO、eNOS水平;取心肌组织,检测ET-1、NO、eNOS表达情况及eNOSmRNA表达情况。结果对照组血及心肌组织中NO、ET-1、eNOS水平及心肌组织中eNOSmRNA表达量分别于8:00及20:00出现峰值,高钠组分别于2:00及14:00出现峰值,高钠组峰值出现时间较对照组提前6 h,相同时点2组血及心肌组织中NO、ET-1、eNOS水平及心肌组织中eNOSmRNA表达量比较差异均有统计学意义(P均<0.05);对照组血钠于2:00出现峰值,高钠组于20:00出现峰值,高钠组较对照组提前6 h,但组内各时点血钠浓度及2组间血钠浓度比较差异均无统计学意义(P均>0.05)。结论高钠饮食使血管内皮功能受损,内皮源性血管活性物质峰值时相提前,高钠饮食可能通过影响内皮源性血管活性物质昼夜节律而对血压昼夜节律产生影响。
Objective It is to observe the changes of the circadian rhythms of blood sodium and the endothelin-1(ET-1),nitric oxide(NO),endothelial nitric oxide synthase(eNOS)and eNOSmRNA in plasma and cardiac tissue of rats under high sodium diet,in order to explore the molecular biological mechanism of abnormal circadian rhythm of blood pressure caused by high sodium diet.Methods A total of 64 SD rats were randomly divided into 2 groups:high sodium group(n=32)drinking 0.9%Nacl saline and control group(n=32)drinking deionized water.After 8 weeks of feeding,8 rats in each group were randomly sacrificed at 2:00,8:00,14:00,and 20:00,respectively,and their blood samples were taken to dectect the levels of blood sodium and plasma ET-1,NO,and eNOS,and the expression of ET-1,NO,eNOS and eNOS mRNA in myocardium tissue were detected respectively.Results In the normal control,both the peak value of NO,ET-1,eNOS in plasma and in myocardium and the eNOSmRNA expression in myocardium were at 08:00 and 20:00 respectively,and that of the high sodium group were at 2:00 and 14:00.The peak time after the high sodium diet was 6 hours ahead of the control group respectively.There were significant differences in the levels of NO,ET-1,eN0S and the expression of eNOS mRNA in myocardial tissue between the two groups at the same time point(P<0.05).The peak value of sodium in plasma in the control group was 02:00,while it was 20:00 after the high sodium.This means that it was 6 hours ahead of the control group,however,there was no significant difference in serum sodium concentration between every time point in each group and between the two groups(P>0.05).Conclusion High sodium diet can induce impaired vascular endothelial function and early peak phase of endothelium derived vasoactive substances.It may affect the circadian rhythm of blood pressure by affecting the circadian rhythm of endothelium-derived vasoactive substances.
作者
王立立
刘苗苗
韩爽
张倩辉
刘美霞
WANG Lili;LIU Miaomiao;HAN Shuang;ZHANG Qianhui;LIU Meixia(Hebei General Hospital,Shijiazhuang 050051,Hebei,China)
出处
《现代中西医结合杂志》
CAS
2019年第27期2985-2988,2992,共5页
Modern Journal of Integrated Traditional Chinese and Western Medicine
基金
河北省医学科学研究重点课题(20170006)
关键词
高钠饮食
内皮源性血管活性物质
昼夜节律
high sodium diet
endothelium-derived vasoactive substance
circadian rhythm
