摘要
为揭示不同氮添加量对贝加尔针茅草原土壤细菌群落结构的影响,依托长期定位试验,采集8个氮添加水平(0、15、30、50、100、150、200、300 kg N·hm^-2·yr^-1)下0~10 cm土层土壤,利用高通量测序技术,分析不同氮添加水平下土壤细菌群落结构特征及其与土壤化学性质的关系。结果表明:随着氮添加水平增加,细菌Alpha多样性指数呈现先增加后降低的趋势,7个氮添加处理的Alpha多样性指数与对照相比无显著差异。在群落中占主导的细菌门类有酸杆菌门(19.01%~38.31%)、变形菌门(6.64%~17.11%)、放线菌门(5.07%~16.06%)、疣微菌门(3.57%~7.40%)、绿弯菌门(3.38%~8.42%)和芽单胞菌门(0.87%~1.81%)。随着氮添加水平的增加,占主导的细菌门类相对丰度表现出不同的变化趋势。氮添加改变了土壤细菌群落结构,且高氮添加对土壤细菌群落结构影响高于低氮添加。相关性分析表明,土壤pH、硝态氮、铵态氮和全磷含量是影响细菌群落结构的主要因素,其中土壤pH对细菌群落结构的影响最大。
This research evaluated the responses of the soil bacterial community structure and diversity to different nitrogen(N)addition levels in Stipa baicalensis steppe.High-throughput sequencing technology was used to study the changes in soil under different N treatments(0,15,30,50,100,150,200,300 kg N·ha^-1·yr^-1).Soil samples were collected from the 0-10 cm soil layer of the eight different N treatments.The Alpha diversity index of the bacterial community was highest in the mid-range of tested N treatments and lower at low and high N rates.There was no significant difference in Alpha diversity index between the seven N treatments and the control.The dominant bacterial phyla were Acidobacteria(19.01%-38.31%),Proteobacteria(6.64%-17.11%),Actinobacteria(5.07%-16.06%),Verrucomicrobia(3.57%-7.40%),Chloroflexi(3.38%-8.42%),Gemmatimonadetes(0.87%-1.81%).The relative abundance of the dominant bacteria and the structure of the soil bacterial community were both affected by the N addition level.The effect of high N addition on the soil bacterial community structure was greater than that of low N addition.A correlation analysis showed that the bacterial community structure was mainly influenced by the pH,nitrate nitrogen,ammonium nitrogen and total phosphorus levels,with pH being the factor most influencing community structure.
作者
刘红梅
杨殿林
张海芳
赵建宁
王慧
张乃琴
LIU Hong-mei;YANG Dian-lin;ZHANG Hai-fang;ZHAO Jian-ning;WANG Hui;ZHANG Nai-qin(Agro-Environmental Protection Institute,Ministry of Agriculture and Rural Affairs,Key Laboratory of Original Agro-Environmental Pollution Prevention and Control,MARA,Tianjin Key Laboratory of Agro-Environment and Agro-Product Safety,Tianjin 300191,China;Agronomy Department of Dezhou University,Department of Ecology and Landscape Architecture,Dezhou 253023,China)
出处
《草业学报》
CSCD
北大核心
2019年第9期23-32,共10页
Acta Prataculturae Sinica
基金
国家自然科学基金面上项目(41877343)资助
关键词
氮添加
贝加尔针茅草原
细菌群落
多样性
高通量测序
nitrogen addition
Stipa baicalensis steppe
bacterial community
diversity
high-throughput sequencing technology
