摘要
该研究探讨雷公藤甲素对艾滋病(acquired immune deficiency syndrome,AIDS)免疫反应生物通路的影响。通过在PubChem数据库中检索雷公藤甲素的靶蛋白,同时在Gene数据库中查找AIDS的相关基因,运用生物信息学分析软件对雷公藤甲素靶蛋白和AIDS的相关基因作用的免疫反应通路进行构图和解析。查找到雷公藤甲素的靶蛋白15个,AIDS相关基因258个。通过分子网络构建,发现雷公藤甲素的靶蛋白与AIDS相关基因存在密切的生物互作关系。在生物通路比对分析中,雷公藤甲素及AIDS共同作用于神经炎症信号通路、Th1和Th2激活途径、模式识别受体在细菌和病毒识别中的作用等21条免疫反应通路,IFNγ,JAK2,NOD1,PTGS2,RORC是雷公藤甲素扰动免疫反应通路的主要分子。IFNγ是雷公藤甲素和AIDS唯一共同作用于这些通路的分子,对AIDS分子有直接或间接的调节作用。该文通过解析中药靶标及疾病基因之间的网络关系及生物功能,可以为雷公藤参与AIDS临床治疗提供有益的证据,推断雷公藤甲素可以通过干预21个免疫反应通路以及IFNγ分子对AIDS起到免疫调节作用。
The aim of this paper was to study the influence of triptolide in the immune response pathways of acquired immune defi-ciency syndrome(AIDS).Target proteins of triptolide and related genes of AIDS were searched in PubChem and Gene databases on line.Molecular networks and canonical pathways comparison analyses were performed by bioinformatics software(IPA).There were 15 targets proteins of triptolide and 258 related genes of AIDS.Close biological relationships of molecules of triptolide and AIDS were es-tablished by networks analysis.There were 21 common immune response pathways of triptolide and AIDS,including neuroinflammation signaling pathway,Th1 and Th2 activation pathway and role of pattern recognition receptors in recognition of bacteria and viruses.Trip-tolide stimulated immune response pathways by the main molecules of IFNγ,JAK2,NOD1,PTGS2,RORC.IFNγis the focus nodes of triptolide and AIDS,and regulates genes of AIDS directly or indirectly.Triptolide may against AIDS by regulating molecules IFNγin immune response pathways.
作者
李立
何小鹃
张弛
赵宁
吕诚
LI Li;HE Xiao-juan;ZHANG Chi;ZHAO Ning;LYU Cheng(Institute of Basic Research in Clinical Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第16期3448-3453,共6页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2008ZX10101001-005-004,Z0613-2)
中国中医科学院基本科研业务费自主选题项目(Z0606)
关键词
雷公藤甲素
艾滋病
免疫反应
triptolide
AIDS(acquired immune deficiency syndrome)
immune response
