摘要
目的建立超高效液相色谱法(ultra performance liquid chromatography,UPLC)测定血浆华法林浓度,探讨心脏换瓣术后患者华法林血药浓度与国际标准化比率(international normalized ratio,INR)测值的关系,以寻求更安全可靠的监测指标,指导临床抗凝治疗的合理用药。方法利用6-甲氧基萘乙酸作为内标,建立一种超高效液相色谱法,测定血浆中的华法林浓度。同时对79例样本的INR、华法林剂量和血浆华法林浓度进行相关性分析。结果华法林和内标的保留时间分别是2.2和1.1min,血浆中华法林的平均提取回收率为96.0%,华法林浓度在15.6~4000ng/ml具有良好的线性(R^2=0.9996),方法回收率为98.5%~99.9%,最低检测限为5.0ng/ml。日内和日间精密度分别低于1.34%和2.69%。79例患者INR和华法林剂量相关性为r^2=0.006(P=0.481),INR和血浆华法林浓度为r^2=0.006(P=0.497),华法林剂量和血浆浓度相关性为r^2=0.298(P=0.001)。结论该方法是一种简便、快速、准确、灵敏的定量测定人体血浆中华法林浓度的UPLC方法,华法林血药浓度测定对于心脏瓣膜置换手术后患者的抗凝治疗具有指导意义。
Objective To establish a method to determine warfarin concentration in plasma by ultra performance liquid chromatography(UPLC),and to explore the relationship between warfarin plasma concentration and international normalized ratio(INR)in patients after cardiac valve replacement,so as to seek more safe and reliable monitoring indicators and guide rational use of anticoagulant therapy.Methods An ultra performance liquid chromatography method has been developed for measuring warfarin concentration in plasma by using 6-methoxy naphthaleneacetic acid(6-MNA)as internal standard(IS).At the some time,the correlation of INR,warfarin dosage and plasma warfarin concentration in 79 samples was analyzed.Results The retention time of warfarin and the IS were 2.2 and 1.1 min,respectively.The average extraction recovery of plasma warfarin was 96.0%,The concentration of warfarin was 15.6~4000 ng/ml with good linearity(R^2=0.9996).The overall accuracy of the method was 98.5%~99.9%and the lowest detection limit was 5.0 ng/ml.The intra-and inter-day variations were less than 1.34%and 2.69%,respectively.The correlation between INR and warfarin dose was r^2=0.006(P=0.481),INR and plasma warfarin concentration was r^2=0.006(P=0.497),and the correlation between warfarin dose and plasma concentration was r^2=0.298(P=0.001)in 79 cases of samples.Conclusions A simple,rapid,accurate and sensitive UPLC method for quantifying warfarin levels in human plasma was validated.The determination of warfarin blood concentration has guiding significance for anticoagulant therapy of patients after heart valve replacement.
作者
唐江涛
陈瑶
白杨娟
苗强
邹远高
Tang Jiangtao;Chen Yao;Bai Yangjuan;Miao Qiang;Zou Yuangao(Department of Laboratory Medicine,West China Hospital of Sichuan University,Chengdu 610041,China;Department of Clinical Laboratory,Women′s and Children′s Hospital of Sichuan Province,Chengdu 610000,China)
出处
《中华临床实验室管理电子杂志》
2019年第3期133-136,共4页
Chinese Journal of Clinical Laboratory Management(Electronic Edition）
关键词
华法林
国际标准化比率
超高效液相色谱法
Warfarin
International normalized ratio
Ultra performance liquid chromatography
