摘要
目的:研究大黄素对烟曲霉菌性角膜炎模型大鼠的抗炎作用机制。方法:建立烟曲霉菌性角膜炎模型,分为模型组和大黄素组,各8只,正常组10只。大黄素组采取大黄治疗,模型组和正常组采取等体积生理盐水治疗。观察大鼠角膜炎症指数、病理学特征,检测肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、细胞间黏附分子-1(ICAM-1)、过氧化物酶体增殖物激活受体(PPAR)、MAPK、NF-κB蛋白。结果:大黄素组大鼠角膜炎症指数、角膜炎症细胞计数、TNF-α、IL-6、ICAM-1水平、MAPK、NF-κB蛋白低于模型组,PPAR蛋白表达高于模型组(P<0.05)。结论:大黄素通过调控PPAR、MAPK、NF-κB蛋白,改善TNF-α、IL-6、ICAM-1炎症因子水平,对烟曲霉菌性角膜炎大鼠起到抗炎作用。
AIM:To study the anti-inflammatory mechanism of emodin in rats with Aspergillus fumigatus keratitis.METHODS:The model of Aspergillus fumigatus keratitis was established and divided into model group and emodin group,8 in each group and 10 in the normal group.The emodin group was treated with rhubarb,and the model group and the normal group were treated with saline of equal volume.Corneal inflammation index and pathological characteristics were observed.Tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),intercellular adhesion molecule-1(ICAM-1),peroxisome proliferator-activated receptor(PPAR),MAPK and NF-κB protein were detected.RESULTS:The corneal inflammation index,corneal inflammatory cell count,TNF-α,IL-6,ICAM-1,MAPK and NF-κB protein in emodin group were lower than those in model group,and the expression of PPAR protein was higher than that in model group(P<0.05).CONCLUSION:Emodin can improve the levels of TNF-α,IL-6 and ICAM-1 inflammatory factors by regulating PPAR,MAPK and NF-κB proteins,and play an anti-inflammatory role in rats with Aspergillus fumigatus keratitis.
作者
勾晓梅
李雪丽
隋源
周丽霞
Xiao-Mei Gou;Xue-Li Li;Yuan Sui;Li-Xia Zhou(Department of Ophthalmology,AVIC Harbin 242 Hospital,Harbin 150066,Heilongjiang Province,China)
出处
《国际眼科杂志》
CAS
北大核心
2019年第9期1466-1469,共4页
International Eye Science
关键词
大黄素
烟曲霉菌
角膜炎
emodin
Aspergillus fumigatus
keratitis
