摘要
目的检测叉头框转录因子3a(FoxO3a)、β-连环蛋白(β-catenin)在胚胎停育绒毛组织中的表达情况,探讨二者在胚胎停育中的相互作用及其与凋亡的关系。方法选取2017年10月-2018年6月就诊于包钢集团第三职工医院妇产科门诊,经彩超动态监测证实为胚胎停育者36例绒毛组织为研究组,同期正常妊娠自愿要求负压吸引术流产者30例绒毛组织为对照组,采用免疫组织化学技术SP法检测FoxO3a和β-catenin在两组中的表达情况。结果与对照组相比,研究组绒毛滋养细胞中的FoxO3a表达降低、β-catenin表达升高,差异有统计学意义(P<0.05),FoxO3a与β-catenin在胚胎停育中的表达呈负相关(P<0.05)。结论胚胎停育滋养细胞中FoxO3a表达降低、β-catenin表达升高,二者可能共同参与胚胎停育的细胞过度凋亡。
Objective To investigate the correlation between the expression of FoxO3a andβ-catenin and apoptosis in the trophoblastic layer of missed abortion.Methods Expressions of FoxO3a andβ-catenin were detected in villi of 30 tissue samples from missed abortion patients in Department of Obstetrics and Gynecology of The Third Staff Hospital of Baogang Group between October 2017-June 2018 by using streptavidin-peroxidase of immunohistochemistry.Another 30 cases from normal early pregnant women were served as a control group.Results The expression level of FoxO3a in the trophoblastic layer of missed abortion group was lower than that in the normal early pregnant group.The expression ofβ-catenin in the trophoblastic layer of missed abortion group was higher than that in the normal early pregnant group.There was a significant correlation between the expression of FoxO3a andβ-catenin in the study group(P<0.05).Conclusion This result suggested that FoxO3a andβ-catenin might be involved in the development of missed abortion.
作者
冯静
赵莉萍
FENG Jing;ZHAO Liping(Department of Obstetrics and Gynecology,The Third Staff Hospital of Baogang Group,Baotou 014010,China)
出处
《中国生育健康杂志》
2019年第5期426-430,共5页
Chinese Journal of Reproductive Health
