摘要
目的建立简便可行的急性肺损伤模型,观察不同时间点伤情变化规律。方法10~12周龄C57BL/6雄鼠36只,采用随机数字表法分为生理盐水组6只和脂多糖(LPS)损伤组30只,LPS损伤组又分为损伤后3d(LPS-d3)、5d(LPS-d5)、7d(LPS-d7)、9d(LPS-d9)、11d(LPS-d11)5组,每组6只小鼠。利用鼻饲法滴注LPS,伤后3、5、7、9、11d取材,通过肺大体观察、计算肺体指数、苏木素-伊红染色(HE染色)病理评分,评估不同时间点急性肺损伤伤情变化情况。结果肺大体观察伤情变化规律表现为LPS-d3伤情最重,肝样变区域占全肺面积35%,LPS-d5、LPS-d7逐渐修复,肝样变区域占全肺面积分别为20%、9%,LPS-d9基本完全修复,双肺无块状肝样变,仅肺门处有红色块状充血,LPS-d11双肺未见明显出血改变,损伤恢复完全。肺体指数:生理盐水组(0.6±0.056),LPS-d3(1.0±0.292),LPS-d5(0.8±0.121),LPS-d7(0.8±0.153),LPS-d9(0.8±0.146),LPS-d11(0.8±0.133);病理评分:生理盐水组(0.2±0.192)分,LPS-d3(20.7±0.577)分,LPS-d5(12.3±2.646)分,LPS-d7(12.4±3.863)分,LPS-d9(3.6±1.678)分,LPS-d11(3.3±2.404)分。LPS损伤组损伤后3d肺体指数、生理盐水组与LPS损伤组损伤后不同天数病理评分比较,差异有统计学意义(P<0.05)。结论利用C57BL/6小鼠通过鼻饲法滴注LPS建立急性肺损伤模型简便可行,急性肺损伤模型,伤情表现为损伤后第3天伤情最重,后逐渐恢复,损伤后第11天基本恢复。
Objective To establish a simple and feasible model of acute lung injury and to evaluate the changing regularity of injury features with time.Methods Totally 36 male C57BL/6 mice(10-12 weeks of age)were randomly divided into saline group(6 mice)and lipopolysaccharides(LPS)injury group(30 mice).The LPS injury group was then equally divided into five sub-groups,i.e.3 days(LPS-D3),5 days(LPS-D5),7 days(LPS-D7),9 days(LPS-D9),and 11 days(LPS-D11)after LPS stimulation,with 6 mice in each sub-group.We used nasal feeding to drip LPS,and collected tissue samples at day 3,5,7,9 and 11 after stimulation,respectively.Lung general observation,lung body index,and Haemotoxylin and Eosin(H&E)staining with pathological scores were performed to evaluate the changing regularity of lung injury over time.Results Lung general observation showed that mice from LPS-D3 had the most severe injuries,with 35%injured parts of the whole lung,which in LPS-D5 and LPS-D7 was 20%and 9%.Those injuries were gradually repaired over time.Lung tissue from LPS-D9 mice showed that most of the mice were recovered from acute lung injury with some congestion remain in the pulmonary hilar.The lung body indexes for each group were as follows:the saline control(0.6±0.056),LPS-D3(1.0±0.292),LPS-D5(0.8±0.121),LPS-D7(0.8±0.153),LPS-D9(0.8±0.146),and LPS-D11(0.8±0.133).The pathological scores of H&E staining were 0.2±0.192,20.7±0.577,12.3±2.646,12.4±3.863,3.6±1.678,and 3.3±2.404 for the saline control,LPS-D3,LPS-D5,LPS-D7,LPS-D9,and LPS-D11 group,respectively.There were statistically significant differences in lung body indexes of LPS-D3,and in pathological scores between the saline control group and LPS injury groups(P<0.05).Conclusion Nasal feeding with LPS is a simple and feasible way to induce acute lung injury in mice model.The most severe injury is observed on the third day after LPS stimulation,and it is recovered completely until the eleventh day.
作者
胡陈
孙剑会
甘乐彬
刘迪
张安强
黄宏
杜娟
文大林
陈民佳
陆红祥
曾灵
张华才
蒋建新
HU Chen;SUN Jian-hui;GAN Le-bin;LIU Di;ZHANG An-qiang;HUANG Hong;DU Juan;WEN Da-lin;CHEN Min-jia;LU Hong-xiang;ZENG Ling;ZHANG Hua-cai;JIANG Jian-xin(State Key Laborotary of Trauma,Burns and Combined Injury,Daping Hospital,Army Medical University,Chongqing 400042,China;Guizhou Medical University,Guiyang 550004,China)
出处
《创伤外科杂志》
2019年第8期601-605,共5页
Journal of Traumatic Surgery
基金
国家自然科学基金资助(81530063)
关键词
肺损伤
肺泡上皮修复
鼻饲法
脂多糖
lung injury
alveolar epithelial repair
nasal feeding
lipopolysaccharides
