摘要
【目的】鉴定来自不同产区分离的桃根癌病病原菌土壤杆菌(Agrobacterium tumefaciens)的致病力。【方法】利用根癌土壤杆菌与T-DNA转移和瘿瘤形成相关的virD2和ipt基因进行分子检测,利用3-酮苷试验等生物学手段对不同地区桃根瘤组织中分离的桃根癌土壤杆菌进行致病性鉴定;并利用指示植物检测不同生物类型菌株的致病能力,最后在不同品种桃植株接种后验证其致病性。【结果】virD2和ipt基因两对特异引物在21株病原菌中均出现203 bp和427 bp的目的片段,表明分离的病原菌均属于根癌土壤杆菌。3-酮苷试验结果表明,有3株菌株由蓝变黄为生物Ⅰ型,其余18株颜色不变,为生物Ⅱ型。指示植物表明,虽然病原菌都能对植株形成瘤,但生物Ⅱ型菌株致病力明显强于生物Ⅰ型,将生物Ⅱ型病原菌接种于不同品种桃树均能长出明显的瘤,也验证了生物Ⅱ型病原菌具有更强致病力。【结论】不同产区分离的桃根癌病病原菌为根癌土壤杆菌,其中多数为生物Ⅱ型,具有强致病力,几乎能侵染所有的桃品种。
【Objective】In this study,the pathogenicity of Agrobacterium tumefaciens,a pathogen isolated from tumor tissues collected from several peach production regions,was identified.【Method】Molecular detection of virD2 and ipt genes associated with A.tumefaciens and T-DNA metastasis and gall tumor formation was carried out.The pathogenicity of A.tumefaciens isolated from tumor tissues collected from different peach production regions was identified by means of the 3-ketoglycosides test and other biological methods.Indicator plants were used to detect the pathogenicity of strains of different biological types.Finally,peach plants of different varieties was used to verify the pathogenicity of crown gall strains by artificial inoculation.【Result】The PCR results showed that there are both 203 bp and 427 bp target fragments of the specific primers virD2 and ipt genes in 21 strains of pathogenic bacteria,Which indicate that the isolated pathogens belong to A.tumefaciens.The results of 3-ketoglycosides test show that 3 strains changed from blue to yellow belong to BiotypeⅠ,and the remaining 18 strains with unchanged color belong to BiotypeⅡ.Indicator plants show that pathogens could form tumors on plants,and the pathogenicity of BiotypeⅡstrains is significantly stronger than that of BiotypeⅠ.The inoculation of BiotypeⅡpathogens in different varieties of peach trees can form tumors obviously,and it also verify that BiotypeⅡpathogens have stronger pathogenicity.【Conclusion】The pathogens of crown gall disease isolated from different peach production areas are A.tumefaciens,most of which belong to Biological biotypeⅡ,which has strong pathogenicity and can infect almost all peach varieties.
作者
郝峰鸽
李桂荣
王保全
蔡祖国
尤杨
牛生洋
HAO Fengge;LI Guirong;WANG Baoquan;CAI Zuguo;YOU Yang;NIU Shengyang(School of Horticulture Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,China)
出处
《广东农业科学》
CAS
2019年第7期86-91,共6页
Guangdong Agricultural Sciences
基金
河南省自然科学基金（182300410076）
河南科技学院高层次人才启动项目（207010616005)
关键词
桃
根癌病
根癌土壤杆菌
接种
生物型
peach
crown gall disease
Agrobacterium tumefaciens
inoculation
biotype
