摘要
为了探索皱皮木瓜(2n=2x=34)多倍体高效诱导体系及再生植株的快速鉴定技术。以皱皮木瓜成熟的胚、当年生实生幼苗、5a生单株的当年生新稍及其腋芽为材料,以不同浓度的秋水仙碱进行不同时间的诱导,基于流式细胞技术对诱导后的再生植株进行倍性鉴定。结果表明:皱皮木瓜四种材料在秋水仙碱诱导下均能产生多倍体,但诱导效率和再生植株的变异率差别显著,其中以5a单株的基部粗度为1.0 cm的新稍为材料,用1.2%秋水仙碱+4.0 mg/L TDZ+2.0 mg/L AgNO3的混合药液处理3 d的诱导体系最佳,其再生植株变异率可达9.09%。经流式细胞仪检测,4 862个再生植株中存在二倍体、三倍体、四倍体和2x/4x嵌合体,其中91个发生变异,变异率为1.87%;三倍体与嵌合体约占变异个数的5.5%。本研究建立一套皱皮木瓜多倍体的高效诱导体系,开创了流式细胞术在木瓜属植株中的应用先河,为皱皮木瓜的种质创新和新品种培育提供科学依据。
In order to develop an effectiv e inducing system for inducement and identification of polyploids in Chaenomeles speciose.Four tissues,such as the mature embryos,stemmed seedlings,current-year-grown branches and axillary buds of diploid C.speciose(2 n=2 x=34)were used to treat with different concentration of colchicine and different treatment duration to induce the polyploid plants.Flow cytometry was used for identification of ploidy in the regeneration plantlets from the colchicine treated seed by determination of their relative DNA contents in leaves.In this study,the results showed that regeneration plantlets were induced from all the four tissues treated by colchicine,but there was significant difference in induction efficiency and total mutation rate among the four tissues.The best inducing system was obtained from the miscible liquids,which were constituted by 1.2%colchicine+4.0 mg/L TDZ+2.0 mg/L Ag NO3,and the current-year-grown branches with 1.0 cm thickness were treated by the miscible liquids for 3 days,and the mutation rate was as high as 9.09%.It was 4 862 regeneration plantlets that contain diploid,triploid,tetraploid and 2 x/4 x chimera.The total number of mutated plantlets were 91 and the mutation rate was 1.87%.Triploid and chimera accounted for 5.5%of the variation.In this study,a set of high efficiency induction system for polyploidy of Chaenomeles speciose was established,and the application of flow cytometry in papaya plants was pioneered,which provided a scientific basis for the breeding of new varieties and germline innovation of Chaenomeles speciose.
作者
刁松锋
姜景民
邵文豪
罗颖
刘金凤
岳华峰
孙晓薇
Diao Songfeng;Jiang Jingmin;Shao Wenhao;Luo Ying;Liu Jinfeng;Yue Huafeng;Sun Xiaowei(Key Laboratory of Non-timber Forest Germplasm Enhancement&Utilization of State Forestry and Grassland Administration,Paulownia Researchand Development Center of State Forestry and Grassland Administration,Non-timber Forestry Research and Development Center of Chinese Academyof Forestry,Zhengzhou,450003;Key Laboratory of Tree Breeding of Zhejiang Province,Institute of Subtropical Forestry Research of Chinese Academyof Forestry,Hangzhou,311400;Henan Academy of Forestry,Zhengzhou,450008)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第17期5754-5762,共9页
Molecular Plant Breeding
基金
中央级公益性科研院所基本科研业务费专项资金(CAFYBB2018SY016
CAFYBB2017MB027)
浙江省公益技术应用研究计划项目(2015C32090)共同资助
关键词
皱皮木瓜
秋水仙碱
多倍体
流式细胞仪
Chaenomeles speciose
Colchicina
Polyploidy
Flow cytometry
