摘要
本研究利用生物信息学方法分析芸薹属物种PDF1.1家族的结构功能,探究芸薹属物种PDF1.1家族的分子进化,为芸薹属物种PDF1.1家族抗菌性提供理论基础。本研究以甘蓝型油菜,白菜,甘蓝为例,利用生物信息学方法,鉴定了这3个芸薹属物种共有8个PDF1.1,并分析其PDF1.1的基本特征、同源性及系统发生关系。结果显示,8个PDF1.1均有1个内含子和2个外显子;共有79～80个氨基酸残基;均具有Knot1结构域,具有多个磷酸化位点,其中丝氨酸磷酸化位点0～1个,苏氨酸磷酸化位点0～1个,酪氨酸磷酸化位点1个;它们的二级结构主要为随机卷曲;既是疏水性蛋白,也是稳定蛋白;在不同物种间芸薹属PDF1.1的同源性高于种内同源性。芸薹属的结构特点是防御素抗菌作用的重要条件之一;其三个物种的PDF1.1进化符合禹氏三角理论,且三个物种PDF1.1位点符合三倍化学说。本研究为芸薹属物种PDF1.1抗菌性提供了科学依据。
This research analyzed the struct ure and function of the PDF1.1 family of Brassica species by bioinformatics,and explored the molecular evolution of the PDF1.1 family of Brassica species,which provided a theoretical basis for the antibacterial properties of the PDF1.1 family of Brassica species.Taking Brassica napus,Brassica rapa and Brassica oleracea as examples,8 PDF1.1,were identified by bioinformatics and their PDF1.1 characteristics,homology and phylogenetic relationship were analyzed.The results showed that all 8 PDF1.1 had one intron and two exons,and there were 79~80 amino acid residues.All of them have Knot1 domain,and have many phosphorylation sites,including one serine phosphorylation site,one threonine phosphorylation site and one tyrosine phosphorylation site,their secondary structures were mainly random crimp.The homology of PDF1.1 in different species is higher than that in species.The structural characteristics of Brassica are one of the important conditions for the antimicrobial action of defensing,an d the PDF1.1 evolution of three species conforms to the theory of Yu’s triangle,and the PDF1.1 loci of three species conform to the triple chemical theory.This study provided a theoretical basis for the antibacterial properties of PDF1.1 family of Brassica species.
作者
向奥玲
张涛
唐蓓
陆俊杏
Xiang Aoling;Zhang Tao;Tang Bei;Lu Junxing(Key Laboratory for Innovative Development and Utilization of Oil Peony Germplasm,Chongqing Engineering Research Center of Specialty Crop Resources,College of Life Sciences,Chongqing Normal University,Chongqing,401331)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第9期2799-2806,共8页
Molecular Plant Breeding
基金
重庆市科学技术委员会基金项目(cstc2017jcyjAX0108
cstc2016shmszx80051)
重庆市教育委员会基金项目(kj1500329)
重庆高校创新团队(CXTDX201601018)共同资助
