摘要
以刚挂果的成龄两性株截顶后产生的侧芽为材料,对“台农杂交2号”番木瓜进行组织培养技术研究。结果表明:外植体经75%酒精、饱和香皂水和300 mg/L利福平预处理后,用75%酒精浸泡50 s、1.5%次氯酸钠10 min和0.1%升汞10 min的复合消毒程序,消毒成功率可提高至80%以上。外植体第2~5个芽位在MS+0.02 mg/L NAA+0.2 mg/L BAP培养基中光照培养4周后出芽诱率可达50%。出芽的外植体在MS+0.02 mg/L NAA+0.2 mg/L BAP+1 mg/L GA3+0.25 mg/L KT培养基中培养4~5个月进行丛生芽诱导,丛生芽诱导后再转接至MS+0.02 mg/L NAA+1 mg/L GA3+0.4 mg/L KT培养基中光照培养1~2月,可获得健壮的、形态正常的不定芽。1.5~2.0 cm的不定芽在MS+0.5 mg/L IBA培养基中暗培养一周后,再转接至1/2 MS与蛭石1:2混合的培养基中培养3周后可形成根系形态正常、发达的完整组培苗,根诱导率达90%以上。
To establish robu st regenerating system of tissue culture for papaya, lateral buds derived from fruit-bearing, hermaphroditic papaya(Carica papaya L.) plants of cv. Tainung No.2 were taken as the explants. The results shows that pretreated with 75% ethanol, saturated toilet soap, 300 mg/L Rifampicin followed by dipping in75% ethanol for 50 s, treating with 1.5% sodium hypochlorite and 0.1% Hg Cl2 for 10 min respectively the disinfection success rate for the explants can be up to 80%. For bud induction, the sterile explants were transferred onto MS medium supplemented with 0.02 mg/L NAA, 0.2 mg/L BAP, 3% sucrose and 0.7% agar for 4 weeks and subcultured monthly on MS medium supplemented with 0.02 mg/L NAA, 0.2 mg/L BAP, 1 mg/L GA3, 0.25 mg/L KT, 3% sucrose and 0.7% agar until multiple bud/shoot clusters formed in 4~5 months. and the multiple bud/shoot clusters were subcultured monthly on MS medium supplemented with 0.02 mg/L NAA, 1 mg/L GA3, 0.4mg/L KT,3% sucrose and 0.7% agar. Shoots 1.5~2.0 cm long were cut from the clusters for root development. During a1-week root-induction stage 0.5 mg/L IBA(MSR1 medium) was used. During a 2-week root-development stage ?liquid basal medium without hormone supported by vermiculite was used.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第12期3479-3482,共4页
Molecular Plant Breeding
基金
国家自然科学基金(31201264)资助
关键词
番木瓜“台农杂交2号”
两性株
侧芽
组织培养
Papaya cv.Tainung No.2
Hermaphroditic papaya
Lateral buds
Tissue culture
