摘要
叶黄素循环在逆境胁迫中对植物光系统起着重要的保护作用。紫黄质脱环氧化酶(VDE)和玉米黄质环氧化酶(ZEP)是调控叶黄素循环的关键酶。本研究采用RT-PCR和RACE技术率先从草莓中克隆获得VDE和ZEP基因。VDE基因c DNA全长1 842 bp,ORF为1 467 bp,编码489个氨基酸;ZEP基因c DNA全长2 325 bp,ORF为1 980 bp,编码660个氨基酸。生物信息学分析表明草莓VDE与ZEP基因所编码的氨基酸序列与蔷薇科的苹果、桃和梅等植物的亲缘关系较近。荧光定量分析发现VDE和ZEP基因在草莓根、茎、叶、花、果实中皆有表达,说明二者均为组成型表达基因,并且叶片中基因的表达量最高而根中最低,推测与其在草莓叶黄素循环调控的作用有关。
The xanthophyll cycle plays an important role in protecting the plant photosystem from adversity stresses. Violaxanthin de-epoxidase(VDE) and zeaxanthin epoxidase(ZEP) are the key enzymes for the xanthophyll cycle. This research firstly cloned the c DNAs of VDE and ZEP in strawberry by RT-PCR and RACE techniques.The full-length c DNA of VDE consists of 1 842 bp with an open reading frame(ORF) of l 467 bp, encoding a polypeptide of 489 amino acids. The full-length c DNA of ZEP consists of 2 325 bp with an open reading frame of1 980 bp, encoding a polypeptide of 660 amino acids. The bioinformatics analysis showed that VDE and ZEP proteins from strawberry had close genetic relationship with rosaceous plants apple, peach and plum. The semi-quantitative RT-PCR analysis showed that ZEP and VDE could be expressed in roots, stems, leaves, flowers and fruits, suggesting that they were constitutive expression in strawberry, and the expression levels of VDE and ZEP were highest in leaves and lowest in roots, which was probably related to their roles in the regulation of xanthophyll cycle for strawberry.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第11期2976-2983,共8页
Molecular Plant Breeding
基金
福建省属公益类科研院所基本科研专项(2014R1026-7
2015R1026-9
2014R1026-6
2015R1026-4)
福建省自然科学基金项目(2015J01118)共同资助
