摘要
本研究采用在山茶属物种表现为单拷贝的RPB2基因12~16及23内含子区域序列,开展浙江红山茶及其8个红山茶组近缘种的分子甄别,以期确认来自不同地理种源的浙江红山茶物种真实性,同时通过与组内几个近缘种的系统发育分析,将浙江红山茶从近缘物种中区分出来。浙江红山茶群体真实性检测结果显示,来自4个地理群体的8个供试材料遗传距离仅为0.001 9~0.006 6,个体间虽已存在一定的分化,但与已知的浙江红山茶聚成一类,而与其他红山茶树种明显区分开来,表明这些供试样品均为浙江红山茶;系统发育分析显示,除滇山茶亚组的多齿红山茶之外,其他供试红山茶组物种能按光果茶亚组与滇山茶亚组分成两个类群,各红山茶组物种的平均遗传距离为0.018 1,表明各物种间亲缘关系较近,其中浙江红山茶与闪光红山茶遗传距离最近仅为0.009 9,而与假多齿红山茶遗传距离最远为0.026 3,分析结果支持将闪光红山茶与浙江红山茶归并一种。本研究拓宽了浙江红山茶种质甄别技术手段,研究结果可为浙江红山茶遗传资源的开发与利用提供技术支撑,同时也可为今后进一步开展红山茶组的分子系统学相关研究奠定方法论基础。
In order to verify the authenticity of Camellia chekiangoleosa from different geographical provenances,and distinguish it from several closely relative species by the phylogenetic analysis, the molecular screening of C.chekiangoleosa and its close relative species was carried out using the introns 12~16 and 23 of the RPB2 gene of genomic DNA. As the results showed, the genetic distance of 8 individuals from 4 geographical populations of C.chekiangoleosa was closely in range from 0.001 9 to 0.006 6. And the differentiation was found in the individuals,but the phylogenetic analysis recovered a monophyletic group of C. chekiangoleosa well separated from the other taxa, which supported the identity of this species. In addition, the phylogenetic system showed that the other Camellia species besides C. polyodonta tested in this study could be divided into two groups according to subsection lucidissima and subsection reticulate. The average genetic distance among Camellia species was 0.018 1, which indicated a close relationship among the species. C. lucidissima was the most similar with C. chekiangoleosa and genetic distance between them was only 0.009 9, while C. chekiangoleosa and C. apolyodenta was the farthest and the distance was 0.026 3, and it might support that C. lucidissima and C. chekiangoleosa should be merged one species group. This study has broadened the technical field for authentication of C. chekiangoleosa and its close relative species, and the r esults could provide technical support for the development and utilization of genetic resources of C. chekiangoleosa, as well as establish the methodological foundation for further molecular system research of sect. Camellia.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第11期2559-2565,共7页
Molecular Plant Breeding
基金
国家自然科学基金项目(31260184)
江西省自然科学基金项目(20151BAB204030)
江西省科技支撑项目(20122BBF60125)
江苏高校优势学科项目(PAPD)共同资助
关键词
浙江红山茶
近缘种
RPB2基因
分子甄别
系统发育
Camellia chekiangoleosa,Relative species,RPB2 gene,Molecular screening,Phylogenetics
