摘要
本研究以‘富士’苹果红色芽变品种‘烟富8’果皮为试验材料,采用RT-PCR方法,克隆获得苹果UV-B受体基因UVR8的全长序列,命名为Md UVR8。结果显示,开放阅读框长度为1 359 bp,编码452个氨基酸,相对分子质量48.487 k D,等电点(PI)为5.56。蛋白质保守域分析表明,苹果UVR8蛋白包含7个RCC1结构域。蛋白质二级结构预测显示,苹果UVR8蛋白含有10个琢-螺旋,16个茁折叠,41个茁-转角。氨基酸同源性比对分析表明,苹果UVR8与已报道的其他植物的氨基酸序列相似性在69.54%~88.94%之间。核苷酸聚类分析表明,苹果和白梨首先聚为一类,其次是梅。本研究为苹果光受体对光应答的分子机理的进一步研究奠定了基础。
This research used‘Yanfu 8'that was red bud mutation variety from‘Fuji'as the experimental material,cloned UV-B receptor gene named Md UVR8 by RT-PCR from fruit peel. The results showed that the full-length of UVR8 gene open reading frame(ORF) was 1 359 bp in size and encoded 452 amino acids residues(Mw=48.487 kD,pI=5.56). Conservative protein domain analysis showed that apple UVR8 protein contains seven RCC1 domain structure,moreover,protein secondary structure prediction revealed that it contains 10 alpha helixes,16 beta folds and 41 beta turns. Amino acids homology comparison analysis indicated that sequence had 69.54%~88.94%similarity with those of other reported plants. Nucleotide cluster analysis showed that UVR8 from Pyrus bretschneideri was clustered together with apple's UVR8 firstly,and followed by which from Prunus mume. This research laid a foundation for further research of the molecular mechanism of photoreceptor response to light in apple.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第8期1779-1785,共7页
Molecular Plant Breeding
基金
公益性行业(农业)科研专项项目(201203075-04)
山东省现代农业产业体系水果创新团队建设基金(SDAIT-03-022-10)共同资助
