摘要
应用随机扩增多态性ＤＮＡ 技术分析１２ 个花生品种的遗传多样性。从８０ 个随机引物中筛选出２０个进行扩增，共扩增出１８０ 条带，其中１３２ 条具有多态性，占７３．３３％ 。平均每个引物提供９ 个ＲＡＰＤ标记的信息量。计算了１２ 个花生品种的遗传相似系数和遗传距离。应用Ｕｎｗ ｅｉｇｈｔｅｄ ｐａｉｒ－ｇｒｏｕｐ ａｖｅｒａｇｅ方法进行聚类分析，结果金花３９—２—１９ 和金花３９—２—５１ 距离最近，并与它们父本泉花１０ 号聚为一类，金花１０１２、金花１０３ 和它们的父本汕油７１ 聚为一类，白皮１ 号与奥油２０２ 聚为一类，而鲁花１４ 和中花２ 号聚为一类，梧油６号，以及ＴＷ０４ 各为一类。
Genetic relationship among peanut ( Arachis hypogaea ) cultivars were analyzed using random amplified polymorphic DNA (RAPD). Of the 80 decamer nucleotide primers screened, 20 showed consistent banding patterns and amplification and produced 180 bands with 132 polymorphic, with an average of 9 bands each primer. The similar indices among the cultivars ranged from 0.6335 to 0.8872. Jinhua 39—2—29 and Jinhua 39—2—51 had the highest similar index of 0.8872, and they were classified into the same group with one of their parent Quanhua 10. The cultivar of TW04 from Taiwan had the lowest similar indices with any other screened cultivars. The result of clustering indicated that RAPD technique was a valuable and reliable method to identify and classify peanut cultivars.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
1999年第3期15-18,共4页
Chinese Journal of Oil Crop Sciences
基金
福建省教委科技基金
