摘要
目的 :用高效液相色谱法测定奥卡西平及片剂的含量和有关物质。方法 :采用DiamonsilC18(2 0 0mm× 4.6mm ,5 μm)色谱柱 ,流动相 :乙腈 0 .0 5mol·L 1磷酸二氢钾 (磷酸调 pH为 3.0 ) (4 0 :6 0 ) ,检测波长 :2 5 6nm ,柱温 :40℃ ,流速 :1.0ml.min 1,峰面积外标法。结果 :在 12 .5～ 112 .5 μg .ml 1的奥卡西平浓度范围内 ,峰面积与浓度呈良好的线性关系 ,回归方程 :Y =3.2×10 5C - 2 .6 1× 10 3 相关系数r=0 .9996 ;片剂中奥卡西平平均回收率为 99.7% (n =5 ) ,RSD =0 .33%。结论 :方法简便 ,快速 ,准确。
OBJECTIVE:To establish a HPLC method for the determination of oxcarbazepine and its tablets and related substances. METHOD:Diamonsil C 18 column (200mm×4.0mm, 5μm), acetonitrile-0.05 mol·L -1 potussium dihydrogen phosphate (adjusted to pH=3.0 by phosphoric acid) (4060) as mobile phase, the detection wavelength was 256nm, column temperature was 40℃, the flow rate was 1.0ml.min -1. RESULTS:The calibration curves was linear in the concentration range of 12.5～112.5μg.ml -1 for oxcarbazepine. The regression equation: Y=3.2×10 5C-2.61×10 3,r=0.9996, the average recovery was 99.7% (n=5), RSD=0.33%.CONCLUSION:This method was found to be simple, quick and accurate for the simultaneous separation and determination of oxcarbazepine and its tablets and related substances.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2002年第5期396-398,共3页
Chinese Journal of Modern Applied Pharmacy
