摘要
目的 :为了开发利用黄芪的抗病毒性质 ,我们进行了此项研究。方法 :我们以阿昔洛韦 (ACV)为阳性对照 ,采用对病毒所致细胞病变的抑制及空斑减数实验 ,观察了黄芪总皂苷、总多糖、总黄酮抗HSV药效。结果 :在Hep - 2细胞系统中 ,ACV对HSV - 1HS - 1株直接杀灭、感染阻断、增殖抑制的ED5 0为 30 .83ug/ml,16 .0 4ug/ml,2 0 .0 4ug/ml;对HSV - 2 333株的相应ED5 0为 16 .45ug/ml,18.6 2ug/ml,10 .85ug/ml.黄芪总皂苷对HSV - 1HS - 1株相应ED5 0为 1.6 8ug/ml,1.72ug/ml,1.95ug/ml;对HSV - 2 333株相应ED5 0为 1.73ug/ml,2 .70ug/ml,2 .74ug/ml.黄芪总多糖对HSV - 1HS - 1株的相应ED5 0为4.0 3ug/ml,5 .33ug/ml,4.90ug/ml;对HSV - 2 333株相应ED5 0为 6 .0 4ug/ml,5 .43ug/ml,7.5 0ug/ml.黄芪总黄酮对HSV - 1HS - 1株的相应ED5 0为 4.95ug/ml,2 .75ug/ml,3.49ug/ml;对HSV - 2 333株的相应ED5 0为 3.5 6ug/ml,3.93ug/ml,6 .2 7ug/m。 结论 :黄芪总皂苷对HSV - 1的治疗指数 (TI)为ACV的 4.4,2 .4,2 .5倍 ;对HSV - 2的治疗指数为ACV的 2 .3,1.0 ,1.6倍。总多糖对HSV - 1的治疗指数为ACV的 10 .3,4.1,5 .5倍 ;对HSV - 2的治疗指数为ACV的 3.7,2 .7,3.3倍。总黄酮的治疗指数与ACV相近。
OBJECTIVE:In order to understand and exploit the antiviral properties of Astragalus membranaceus. METHOD:ACV was used as positive control of antiherpes,through inhibitions of cellular pathological effects of herpes simplex virus type-2 and plaque reduction assays,the effects of antiherpes of total saponins ,total polysaccharieds,total flavonoids of Astragalus membranaceus were observed. RESULTS:In Hep-2 cell systems,the median effect doses(ED50) of ACV of direct virucidal activity,the inhibition effects on the infection and multiplication of HSV-1 HS-1 strain were 30.83ug/ml,16.04ug/ml and 20.04ug/ml, respectively the corresponding figures to HSV-2 333 strain were 16.45ug/ml,18.62ug/ml and 10.85ug/ml, respectively; the corresponding ED50s of total saponins to HSV-1 HS-1 strain were 1.68ug/ml,1.72ug/ml and 1.95ug/ml,respectively; those to HSV-2 333 strain were 1.73ug/ml,2.70ug/ml and 2.74ug/ml, respectively; the corresponding ED50s of total polysaccharides to HSV-1 HS-1 strain were 4.03ug/ml,5.33ug/ml and 4.90ug/ml, respectively; those to HSV-2 333 strain were 6.04ug/ml,5.43ug/ml and 7.50ug/ml, respectively.The corresponding ED50s of total flavonoids to HSV-1 HS-1 strain were 4.95ug/ml,2.75ug/ml and 3.49ug/ml, respectively; to HSV-2 333 strain were 3.56ug/ml,3.93ug/ml and 6.27ug/ml, respectively. CONCLUSION:The Treatment Indices(TI) of total saponins to HSV-1 HS-1 strain were 4.4,2.4 and 2.5times of ACVTIs,to HSV-2 333 strain were 2.3,1.0 and 1.6 times of ACVTIs.The TIs of total polysaccharides to HSV-1 HS-1 strain were 10.3,4.1 and 5.5 times of ACVTIs;to HSV-2 333 strain were 3.7,2.7 and 3.3 times of ACVTIs.The TIs of total flavonoids were close to ACVTIs.These components of Astragalus membranaceus should be worth exploitation.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2002年第5期356-359,共4页
Chinese Journal of Modern Applied Pharmacy
基金
湖北省自然科学基金资助
关键词
黄芪
实验研究
黄芪总皂苷
黄芪总多糖
黄芪总黄酮
抗病毒
人疱疹病毒
Total saponins, total polysaccharides, total flavonoids of Astragalus membranaceus, antivirus, herpes simplex virus
