摘要
为全面了解湖南稻瘟病菌群体遗传的多样性 ,为水稻育种和品种抗性基因的合理布局提供充分的理论依据 ,用 Pot2 Rep- PCR对 2 0 0 1年采自并分离的湖南晚稻和一季稻 41个品种的 12 9个单孢菌株进行了 DNA指纹分析 .结果表明 ,以 72 %相似水平 ,可将 12 9个菌株分成 4个谱系 ,2 4个单元型 .优势谱系为 L1和 L3,它们分别拥有6个和 15个单元型 ,其菌株数分别占总数的 41.0 9%和 46 .5 1% .优势单元型为 H5 ,H6和 H17,分别占总菌株数的16 .2 8% ,16 .2 8%和 17.83% .研究揭示稻瘟病菌存在较大的变异潜能 ,稻瘟病菌的群体遗传多样性与特定地区水稻品种组成的多样性呈密切的正相关关系 .
A total of 129 monoconidial isolates of Magnaporthe grisea was collected and isolated from 41 rice cultivars or lines, which are late and single rice in Hunan. Each isolate was subjected to DNA fingerprinting analysis by Pot2 Rep-PCR. At 72% similarity level, these isolates can be grouped into 4 lineages,and composed of 24 haplotypes,as the result shows. Of these lineages, L1 and L3 were dominate lineages with 6 and 15 haplotypes,whose total 41.09% and 46.51% respectively. The dominate haplotypes were H5, H6 and H17 which covered 16.28%,16.28% and 17.83% of the total isolates separately.The study reveals that there is rather heterogeneous and potential variation in the blast pathogen, and a close positive relationship between genetic diversity of pathogen population structure and the diversity of cultivar constitutes.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第5期391-394,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
亚华种业股份有限公司博士后基金资助项目
