摘要
目的 通过动物在体实验,筛选柔毛霉素抑制后囊膜混浊眼前段用药的安全有效剂量。方法 在家兔眼晶状体囊外摘出术中联合不同质量浓度的柔毛霉素(5～20 mg/L)囊袋内灌注10 min,通过对术后1周各质量浓度组角膜内皮细胞、虹膜、睫状体组织反应的光、电镜检查和术后1个月各组晶状体前囊膜上皮细胞密度差异的观察,分别探讨药物在前房应用的安全、有效剂量。结果5～20 mg/L均可有效抑制晶状体前囊膜上皮细胞的增殖,呈一定的质量浓度依赖性。5 mg/L柔毛霉素可有效抑制晶状体前囊膜上皮细胞的增生且无临近组织的损伤,10～20 mg/L可引起角膜内皮细胞亚细胞水平可逆性损伤和虹膜睫状体急性炎症反应;20 mg/L可引起眼表组织损伤。 结论 5 mg/L柔毛霉素是抑制后囊膜混浊前房用药的安全有效剂量,具有临床应用价值和可行性。
Objective To investigate the safe and effective concentration of daunomycin as a preventer of posterior capsule opacification (PCO) in animal experiments. Methods 5 ~ 20 mg/L daunomycin was infused into lens capsular bag for 10 minutes during ECCE operation on rabbits eyes. At the 1st week after operation, response of corneal endothelial cells, iris and ciliary body were observed with light and electron microscope. 1 month later,the difference of cell density in the lens epithelium of each group exposed to different drug concentration was studied. The safe and effective dose of daunomycin into the anterior chamber was obtained. Results The concentrations of 5 ~ 20 mg/L could effectively inhibit the proliferation of the anterior capsular epithelium in a dose-dependent manner. 5 mg/L of daunomycin could effectively inhibit the proliferation of the anterior capsular epithelium without toxicity to adjacent ocular tissues. The concentrations of 10 -20 mg/L could cause microchanges in corneal endothelial cell and acute inflammation of iris and ciliary body;20 mg/L daunomycin could damage ocular surface tissues. Conclusion The safe and effective concentration of daunomucin in preventing the posterior capsule opacification is 5 mg/L. This dose may be valuable for the prevention of PCO during clinical application.
出处
《眼科研究》
CSCD
北大核心
2002年第6期520-523,共4页
Chinese Ophthalmic Research
