摘要
目的建立一测多评法(QAMS)同时测定自制雷公藤提取物6种有效成分的含量。方法采用QAMS法,以雷公藤甲素、雷公藤内酯酮、雷酚内酯、雷公藤次碱、雷公藤红素和雷公藤内酯甲6种成分为研究对象,对外标法和校正因子法的实测值及计算值的差异进行分析。结果 6种指标成分在一定范围内线性关系良好,以雷公藤甲素为内参物,雷公藤内酯酮、雷酚内酯、雷公藤次碱、雷公藤红素和雷公藤内酯甲的fk/s分别为0.654、1.221、0.347、1.238、0.079,且在不同条件下重复性良好;3批样品中各成分含量分别为雷公藤甲素0.037～0.038mg/g、雷公藤内酯酮0.245～0.253 mg/g、雷酚内酯6.165～6.325 mg/g、雷公藤次碱14.439～15.065 mg/g、雷公藤红素1.699～1.856 mg/g、雷公藤内酯甲20.946～21.825 mg/g,且计算所得量与实测量测得结果无显著差异。结论QAMS法适用于雷公藤提取物的含量测定,可为雷公藤的质量控制提供参考。
Objective: To establish a multiple evaluation method(QAMS) to determine the content of 6 active ingredients of Tripterygium wilfordii extract including triptolide, triptonide, triptophenolide, wilfordine, celastrol and wilforlide A. Methods: 6 components were studied by QAMS method. The difference between the measured values and the calculated values of the external standard method and the correction factor method was analyzed. Results: The 6 indexes had good linear relationship in a certain range,and the fk/s of them were 0.654, 1.221, 0.347, 1.238, 0.079, respectively. The reproducibility was good under different conditions,and the contents of 3 batches of samples were 0.037~0.038 mg/g triptolide, 0.245~0.253 mg/g triptonide, 6.165~6.325 mg/g triptophenolide, 14.439~15.065 mg/g wilfordine, 1.699~1.856 mg/g celastrol and 20.946~21.825 mg/g wilforlide A, and there was no significant difference between the calculated amount and the measured results. Conclusion: A multiple evaluation method is applicable to the content determination of Tripterygium wilfordii extract, which can provide reference for the quality control of Tripterygium wilfordii.
作者
吴珏
刘志宏
林兵
张敏新
林莉莉
周桂芝
宋洪涛
WU Jue;LIU Zhihong;LIN Bing;ZHANG Minxin;LIN Lili;ZHOU Guizhi;SONG Hongtao(College of Pharmacy,Fujian University of Chinese Traditional Medicine,Fuzhou,Fujian 350122,China;900 Hospital of the Joint Logistics Team,Fuzhou,Fujian 350025,China)
出处
《福建中医药》
2019年第1期32-35,共4页
Fujian Journal of Traditional Chinese Medicine
基金
福建省自然科学基金项目(2017J05135)
福州总医院院立课题(2016L02)
关键词
雷公藤
一测多评法
相对校正因子
QAMS
Tripterygium wilfordii
relative responding factor
