摘要
研究了草莓和番茄果实采后在 4℃和 2 0℃下 ,NAD激酶 (NADK)、NADP磷酸酶活性变化及其与果实呼吸、乙烯释放率、钙调素拮抗剂三氟啦嗪 (Trifluoperazine ,TFP)的关系。结果表明 ,两种果实的NADK、NADP磷酸酶活性变化趋势存在显著差异 ,草莓果实采后NADK活性先下降、后上升 ,NADP磷酸酶活性出现第 1个峰后继续缓慢上升 ,而番茄果实NADK活性先上升、后下降 ,NADP磷酸酶活性在出现第 1个峰后继续下降 ;两种果实NADK活性变化与呼吸变化趋势相似 ,NADP磷酸酶变化与乙烯释放率变化趋势相似 ;低温激活NADK ,抑制NADP磷酸酶 ,TFP对NADK有激活作用 ,对NADP磷酸酶影响较小。表明果实后熟衰老过程与NAD激酶、NADP磷酸酶密切相关 ,激活NADK可能与延缓果实后熟衰老有关。
Activities of NAD kinase and NADP phosphatase and relationship between the two enzymes and temperature, respiration, ethylene production and Trifluoperazine in postharvested strawberry and tomato fruits under 4℃and 20℃ were studied. The activity of NAD kinase in strawberry decreased slowly during first four days, then increased gradually. The NADP phosphatase activity increased at the second day after harvest, decreased the next day, then increased again. For tomato fruits, the activity of NAD kinase and NADP phosphatase increased at the second day after harvest, decreased with the ripening and senescence. The changing model of NADK and respiration in two fruits were similar, the same were NADP phosphatase and ethylene production. TFP enhanced the activity of NADK and had little effect on NADP phosphatase. Low temperature(4℃) activated the NADK and reduced the activity of NADP phosphatase. These results indicated that the NAD kinase and NADP phosphatase were related with the ripening and senescence of strawberry and tomato fruits. The activation of NAD kinase probably postponed the ripening and senescence of fruit.
出处
《中国农业科学》
CAS
CSCD
北大核心
2002年第11期1381-1384,共4页
Scientia Agricultura Sinica
基金
河北省农林科学院博士资金资助项目
