摘要
目的 :探讨HLA—Ⅱ类区的 (DQAl、DR2、DR3、DR4、DR7、DR9) 6个基因位点在中国人群肠型胃癌中的突变发生情况 ,进而探讨其是否与胃癌的发生易感性有关。方法 :采用PCR系列技术 (SSP -PCR和PCR/SS CP)。结果 :HLA -Ⅱ类基因区 6个基因位点基因型在个体中存在着明显多态性 ,PCR -SSCP结果显示DR4基因位点突变频率为 45 % ,明显高于其它几个基因位点。结论 :HLA—Ⅱ类基因区DR4基因的突变在国人肠型胃癌组织中较多见 。
Objective:To determine the mutation of HLA-Ⅱ loci DQA1, DR2, DR3,DR4, DR6, DR7 and DR9 in intestinal type gastric carcinoma and detected the relation between the aberration of these HLA-Ⅱ loci and tumor susceptibility. Methods:By performing analysis of SSP-PCR and PCR/SSCP techniques. Results: The mutation incidence of HLA-DR4 loci is 9/20(45%), higher than the other loci in HLA-II. Conclusion: The mutation of the HLA-Ⅱ loci DR4 is closely related to intestinal type gastric carcinoma.
出处
《内蒙古医学院学报》
2002年第2期77-81,共5页
Acta Academiae Medicinae Neimongol
