摘要
AIM：Toinvestigate the effects of vitamin E succinate(VES)on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells.METHODS:After SGC-7901cells were treated with VESat different doses(5,10,20mg·L^-1)at different time,reverse transcription-PCR technique was used to detect the level of c-jun mRNA;Western Blot was applied to measure the expression of c-jun protein.RESULTS:After the cells were theated with VESat 20mg·L^-1for 3h,the expression rapidly reached its maximun that was3.5times of UT control(P<0.01).The level of c-jun mRNA was also increased following treatment of VESfor 6h.However the expression after treatment of VES at 5mg·L^-1for24h was 1.6tmes compared with UTcontrol(P<0.01),Western blot analysis showed that the level of c-jun protein was obviusly elevated in VES-treated SGC-7901cells at 20mg·L^-1for3h,The expression of c-jun protein was gradually increased after treatment of VES at 20mg·L^-1for3,6,12and24h,respectively,with an evident time-effect relationship.CONCONCLUSION:The levels of c-jun mRNA and protein in VES-treated SGC-7901cells were increased in a dose-and time-dependent manner;the expression of c-jun was prolonged by VES,indicating that c-jun is involvedin VES-induced apoptosis in SGC-7901 cells.
AIM:To investigate the effects of vitamin E succinate(VES) on the expression of c-jun gene and protein in human gastric cancer SGC-7901 cells. METHODS:After SGC-7901 cells were treated with VES at different doses(5,10,20 mg·L^(-1))at different time,reverse transcription-PCR technique was used to detect the level of c-jun mRNA;Western Blot was applied to measure the expression of c-jun protein. RESULTS:After the cells were treated with VES at 20 mg·L^(-1) for 3 h,the expression rapidly reached its maximum that was 3.5 times of UT control(P<0.01).The level of c-jun mRNA was also increased following treatment of VES for 6 h. However,the expression after treatment of VES at 5 mg·L^(-1) for 24 h was 1.6 times compared with UT control(P<0.01). Western blot analysis showed that the level of c-jun protein was obviously elevated in VES-treated SGC-7901 cells at 20 mg·L^(-1)for 3 h.The expression of c-jun protein was gradually increased after treatment of VES at 20 mg·L^(-1)for 3,6,12 and 24 h,respectively,with an evident time-effect relationship. CONCLUSION:The levels of c-jun mRNA and protein in VES-treated SGC-7901 cells were increased in a dose-and time-dependent manner;the expression of c-jun was prolonged by VES,indicating that c-jun is involved in VES- induced apoptosis in SGC-7901 cells.
基金
National Natural Science Foundation of China,No.39870662
