摘要
Purpose:To evaluate the expression patterns of several keratin proteins in pteygial epithelium.Methods:The proteins of K8,K10,K14,K16andAE3 in the epithelium of 8pterygia and 4normal conjunctiva were detected using immunofluorescent staining.Results:In pterygial epithelium,the staining patterns of 5keratin proteins were different with normal conjunctiva.No staining of K8,K10and K16was detected in normal conjunctival epithelium,but in pterygial epithelium,K8and K16were present in full thickness and K10was present in superficial cells.In normal conjunctiva.only base layer of epithelium showed positive staining with antibody of K14and AE3proteins,but they were present in the full thickness in pterygial epithelium and the antibody of AE3 protein stained the superficial cells heavily comparing with base cells.Conclusion:The Abnormal expression of keratin proteins(K8,K10,K14,K16andAE3)in pterygia indicates that the epithelial cells in pterygia are in the state of hyperproliferation and keratinization,which may,be the cause of the abnormal tear function in patients with pterygia,Eye Science2000;16:48-52.
Purpose: To evaluate the expression patterns of several keratin proteins in pterygialepithelium.Methods:The proteins of K8, K10, K14, K16 and AE3 in the epithelium of 8 pterygiaand 4 normal conjunctiva were detected using immunofluorescent staining.Results: In pterygial epithelium, the staining patterns of 5 keratin proteins weredifferent with normal conjunctiva. No staining of K8, K10 and K16 was detected innormal conjunctival epithelium, but in pterygial epithelium, K8 and K16 were present infull thickness and K10 was present in superficial cells. In normal conjunctiva, only baselayer of epithelium showed positive staining with antibody of K14 and AE3 proteins, butthey were present in the full thickness in pterygial epithelium and the antibody of AE3protein stained the superficial cells heavily comparing with base cells.Conclusion: The abnormal expression of keratin proteins (K8, K10, K14, K16 andAE3) in pterygia indicates that the epithelial cells in pterygia are in the state ofhyperproliferation and keratinization, which may be the cause of the abnormal tearfunction in patients with pterygia. Eye Science 2000; 16: 48 - 52.
出处
《眼科学报》
2000年第1期48-52,共5页
Eye Science
