摘要
应用已建立的检测MG和MS的PCR方法 ,对人工接种鸡毒支原体后 2～ 2 0天的SPF鸡气管棉拭样品和气管、肺、肝、脾、胸肌、腿肌等器官和组织进行了检测 ;对现场采集的样品同样作PCR检测。结果表明 ,上述人工样品均检测到病原 ,以气管棉拭样品检出最多 ;现场样品PCR阳性检出率为 10 .2 8% ,分离培养的阳性率为 2 .8% ,阳性符合率为 10 0 %。
SPF chickens were infected with Mycoplsma Gallisepticum by inoculation of trachea.The samples of trachea swab,trachea,lung,liver,spleen,chest muscle and leg muscle in SPF chicken were collected from 2 to 20 days postionculation.The clinical samples were also collected.MG and MS in all samples were detected by PCR.Results show that MG positive rate in the trachea swab of artificial samples more than that in other tissues.The MG positive rate detected by PCR in clinical samples is 10.28%,the MG positive rate detected by culture method is 2.8%.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2002年第6期477-479,共3页
Chinese Journal of Preventive Veterinary Medicine
关键词
PCR技术
鸡
毒支原体感染
病原检测
Polymerase Chain Reaction
Mycoplasma Gallisepticum
Sample
