摘要
从组织块长出的人支气管上皮细胞培养于MCDB151无血清培养基中,利用非程序性DNA合成(UDS)和微核试验检测化学致癌物和X射线对人支气管上皮细胞的遗传毒理作用。直接作用致癌物MNNG对所测试的7例上皮细胞均可分别引起UDS或微核增高,并具有剂量反应关系。X射线对4例都可引起微核增高。然而间接作用致癌物NNK和BaP引起的反应有明显个体差异。UDS试验中,NNK处理5例中有3例增高,BaP处理的4例中有3例增高。微核试验表明NNK处理的5例中3例为阳性,BaP处理4例中3例为阳性。本实验结果提示,NNK,BaP和X射线可能是人支气管上皮细胞潜在的致癌物。
Human bronchial epithelial cells were obtained from outgrowth of ex-plants and cultured in serum-free MCDB 151 medium. UDS and micronucleus test were used in this experiment to observe genotoxic effect of chemical carcinogens and x-ray on human bronchial epithelial cells.The direct-acting carcinogen MNNG induced both UDS and micro-nuclei with dose response in all 7 cases examimined separately. Significant increase of micronuclei were found in all 4 cases treated with x-ray. However, there was an individual variation in response to indirect-acting carcinogens NNK and BaP. Significant Increases of UDS were noted in 3 out of 5 cases for NNK treatment, and 3 out of 4 cases for BaP. In micronucleus test, positive results were observed in 3 out of 5 cases for NNK, and 3 out of 4 cases for BaP. Our experimental results indicated that NNK, BaP and x-ray are potential carcinogens to human bronchial epithelial cells
出处
《癌变．畸变．突变》
CAS
CSCD
1992年第6期1-4,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
致癌物
X射线
上皮细胞
遗传毒理
Human bronchial epithelial cells
cancinogen
Unscheduled DNA synthesis
micronucleus test
