摘要
本文利用蚕豆根尖细胞微核测试法,研究了硒(Se)与镉(Cd)的致突变效应及其致突变效应的拮抗作用。结果表明,硒与镉(浓度均分别为5、10、15、20、25和30微克/毫升)均能使蚕豆根尖细胞微核率极显著地高于阴性对照组(P<0.001),硒的剂量效应呈非直线关系,以浓度为20微克/毫升时最高,镉的剂量效应关系呈直线回归,回归方程为Y=0.8X+1.1并且发现两种金属元素在致突变效应方面具有拮抗作用,硒/镉比值(亚硒酸钠与氯化镉的摩尔浓度之比)以1:4时拮抗作用最强。结果提示;硒与镉拮抗作用的机制可能与两种溶液混合后形成的亚硒酸镉的白色絮状沉淀有关,详细机制需进一步探讨。拮抗作用的强弱主要取决于硒/镉比值,同时与硒和镉的浓度有关。
The mutagenic effect and antagonism of Se and Cd were studied using the micronuclear fre quency (MNF) test of root tip cell of broad bean in this article. The results indicated that the MNFs of Se and Cd were significantly higher than that of negative control in all the concentrations (5, 10, 15, 20, 25 and 30μg/ml) tested (P<0. 001). The dose-effect relationship of Se was non-linear, but Cd was linear, with regression equation Y=0.8X+ 1. 1. It was also discovered that this two metals were antagonistic in mutagenic effect. The antagonism was most significant when the ratio of Se/Cd (the mole density ratio of Na2SeO3 and CdCl2 solution) was 1 : 4. While the extent of antagonism was mainly determined by the ratio of Se/Cd, it also related to the concentrations of Se and Cd.
出处
《癌变．畸变．突变》
CAS
CSCD
1992年第5期19-21,14,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
硒
镉
致突变
拮抗作用
Selenium
Cadmium
Mutagenic effect
antagonism
