摘要
为建立一种高效、准确鉴定烟草品种抗赤星病的方法,以4种不同抗赤星病的烟草品种为材料,研究了在不同浓度赤星病菌AT-毒素培养基上处理10、15、20d对烟草种子萌发和根系生长的影响。结果表明,制备的AT-毒素原液中蛋白质、可溶性糖质量浓度分别为80.52μg/mL和23.39mg/mL。随AT-毒素胁迫浓度的增大,烟草种子的发芽率和根系扎进培养基的株数比率均呈下降趋势。在一定AT-毒素浓度和胁迫时间范围内,不同品种对AT-毒素胁迫的耐受力表现出显著差异。在50%AT-毒素胁迫15和20d时,抗病品种净叶黄根系扎进培养基中的株数比率分别为G28、YN116、红花大金元的1.18、1.75、6.02倍和1.02、1.75、5.52倍。综合分析,以烟草种子根系扎进培养基的株数比率作为鉴定烟草品种是否抗赤星病比发芽率更为准确,其最佳AT-毒素浓度为50%、最佳胁迫时间为15~20d。
In order to establish an efficient and accurate method to identify the resistance of tobacco varieties against tobacco brown spot, four tobacco varieties with different resistance levels were used to study the effects of different concentrations of AT-toxin from Alternaria alternate on tobacco seed germination and seedling root growth after treating for 10, 15 and 20 d. The results showed that the protein and soluble sugar contents in AT-toxin solution were 80.52 μg/mL and 23.39 mg/mL, respectively. With the increase of AT-toxin concentration, the germination rate of tobacco seeds and the percentage of tobacco seedlings with roots’growing into AT-toxin medium (PTS) decreased. Within the AT-toxin concentration and treatment time ranges, the tolerances of tobacco varieties to AT-toxin differed significantly. Comparing with G28, YN116 and Honghuadajinyuan, the PTS of Jingyehuang (resistant variety) were 1.18, 1.75, 6.02 times and 1.02, 1.75, 5.52 times in the 15th and 20th days after 50% AT-toxin treatment, respectively. Through the comprehensive analysis shown, PTS as an indicator of whether tobacco varieties resistant to brown spot is more accurate than seed germination rate, the optimal AT-toxin concentration is 50% and the optimal treatment time is 15-20 d.
作者
卢小亮
张强
孙渭
陈鑫
吴洪启
陈耀锋
LU Xiaoliang;ZHANG Qiang;SUN Wei;CHEN Xin;WU Hongqi;CHEN Yaofeng(College of Agronomy,Northwest A&F University,Yangling 712100,Shaanxi,China;Shaanxi Province Tobacco Corporation of CNTC,Xi’an 710004,China)
出处
《烟草科技》
EI
CAS
CSCD
北大核心
2019年第10期22-27,共6页
Tobacco Science & Technology
基金
中国烟草总公司陕西省公司2016年科技计划项目“秦巴烟区优质烤烟新品种选育与区试”（KJ-2016-01）
关键词
烟草品种
赤星病
AT-毒素
抗性鉴定
Tobacco variety
Brown spot
AT-toxin
Resistance identification
