摘要
从山东省临沂市兰陵县、山东省寿光市、河南省商丘市夏邑县多年种植白菜且根肿病爆发严重地块采集正常白菜植株根际周围土样,对其进行生防菌分离筛选及防治白菜根肿病效果评价,以期获得高效拮抗白菜根肿病菌的生防菌。通过梯度平板稀释法对采集土样进行生防菌分离,以黄瓜立枯病菌和香蕉枯萎病菌为指示菌对生防菌进行初步筛选,利用根肿病孢子裂解试验进一步筛选,通过盆栽试验评价生防菌株防治根肿病的效果。结果表明,放线菌株KA-35对白菜根肿病的防治效果为57.11%,单株地上部鲜重增加127.40%。该研究筛选出了对白菜根肿病有良好生防效果的菌株,为生防菌的进一步研究工作奠定了基础。
Soil samples around the rhizosphere of normal Chinese cabbage plants were collected from Lanling County of Linyi City and Shouguang City in Shandong Province and Xiayi County of Shangqiu City in Henan Province,where the outbreak of Clubroot disease was serious for many years.The isolation and screening of biocontrol bacteria and the evaluation on the control effect were carried out in order to obtain biocontrol bacteria with high efficiency against Clubroot disease of Chinese cabbage.The gradient plate dilution method was used to isolate biocontrol bacteria from soil samples.The biocontrol bacteria were preliminarily screened out by plate confrontation test with Cucumber Rhizoctonia solani and Banana Fusarium wilt as indicator bacteria.The spore lysis test of Clubroot disease was conducted for further screening.Finally,the effect of biocontrol bacteria screened out by pot experiment was evaluated.The results showed that the control effect of actinomycete KA-35 on Clubroot disease was 57.22%in pot experiment,and the aboveground fresh weight per plant increased by 127.40%.The strains with better biocontrol effects against Chinese cabbage Clubroot disease were screened out,which laid a foundation for further research.
作者
刘振香
顾志光
徐文凤
孟祥坤
胡兆平
胡红涛
Liu Zhenxiang;Gu Zhiguang;Xu Wenfeng;Meng Xiangkun;Hu Zhaoping;Hu Hongtao(Kingenta Ecological Engineering Group Co.,Ltd.,Linyi 276700,China;State Key Labortory of Nutrition Resources Integrated Utilization,Linyi 276700,China;Key Laboratory of Plant Nutrition and New Fertilizer R&D,Ministry of Agriculture,Linyi 276700,China)
出处
《山东农业科学》
2019年第10期130-134,共5页
Shandong Agricultural Sciences
基金
山东省科技重大创新工程项目“设施蔬菜水肥精准施用关键技术研究与示范”(2017CXGC0205)
关键词
白菜
根肿病
生防菌
防治效果
Chinese cabbage
Clubroot disease
Biocontrol bacteria
Control efficiency
