摘要
目的:建立三痹汤中主要成分龙胆苦苷含量测定的方法,为三痹汤的质量控制提供实验依据。方法:采用HPLC-DAD法测定三痹汤中龙胆苦苷的含量,仪器为安捷伦1260infinityⅡ,色谱柱为Inetsil-ODSC18(4.6mm×250mm,5μm),流动相为乙腈(A)-0.05%磷酸水溶液(B)进行梯度洗脱,流速为1mL/min,检测波长为250nm。结果:该方法的精密度,稳定性符合要求,主要成分的峰面积和质量浓度之间的线性关系良好,r大于0.999,平均加样回收率99.8%~100.0%,RSD小于0.1%,龙胆苦苷的仪器检测限为0.005483μg/mL,定量限为0.01845μg/mL。结论:本文建立了三痹汤的HPLC主要成分龙胆苦苷含量测定方法,可以为三痹汤质量控制提供实验依据参考。
Objective: To developa method for determination of gentiopicroside in San Bi Tangextracts,provide experimental basis for quality control of San Bi Tanggranules.Methods:In this study, the gradient elution of acetonitrile (A)- water (B) was carried out by using the method of HPLC-DAD with Agilent 1260 infinity II as the instrument, Inetsil- ODS C18 (4.6 mm×250 mm, 5 μm) as the column, and the mobile phase was acetonitrile (A)- water (B). The flow rate was 1 mL/min, and the detection wavelength was 250 nm.Results:The precision and stability of the method meet the requirements. The linear relationship between peak area and mass concentration of main components is good, R is greater than 0.999. The average recovery of sample addition is 99.8%~ 100.0%, RSD is less than 0.1%, the detection limit of gentiopicroside is 0.005483μg/mL, and the quantitative limit is 0.01845μg/mL.Conclusions: A method for the determination of gentiopicrosidein the extract of San Bi Tang has been established, which can provide experimental basis for the quality control of San Bi Tang.
作者
但宇超
曾琪
马安献
张惠芹
季宗念
严启新
Dan Yu-chao;Zeng Qi;Ma An-xian;Zhang Hui-qin;Ji Zong-nian;Yan Qi-xin(HaiwangBaicaotang Pharmaceutical Co., Ltd., Henan Puyang 457000)
出处
《生物化工》
2019年第5期24-26,38,共4页
Biological Chemical Engineering
基金
河南省中药饮片质量控制工程技术研究中心(豫科[2016]221号)
2018年度濮阳市重大科技专项
2017年河南省科技厅科技攻关项目
