摘要
目的建立定量检测血浆甲磺酸伊马替尼的液相色谱-串联质谱(LC-MS/MS)方法,并对此方法进行分析性能验证.方法血浆经蛋白沉淀法处理后,使用LC-MS/MS的多反应检测模式(MRM)检测,监测离子对为m/z 494.3→m/z 394.2(伊马替尼)、m/z237.3→m/z194.1(卡马西平,内标).结果 LC-MS/MS检测血浆甲磺酸伊马替尼的线性范围为6.25~3 200.00 μg/L,批内、批间精密度分别为1.98%~4.46%、3.07%~ 7.54%,正确度偏倚为-7.34%~2.28%,基质效应因子在0.93~1.05.结论建立的LC-MS/MS性能符合评价标准,灵敏度高,结果准确、稳定,可用于临床分析.
Objective To establish a liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitation determination of plasma imatinib mesylate and perform the general method Validation. Methods After proteins in plasma were precipitated, imatinib was analyzed by LC-MS/MS mode and multiple reaction monitor (MRM) mode, the ion pairs monitored were m/z 494.3→m/z 394.2 (imatinib), m/z237.3→m/z194.1 (carbamazepine, internal standard). Results The linear range was 6.25 to 3 200.00 μg/L. The within-run precision was 1.98%-4.46%, and between-run precision was 3.07%-7.54%. The correctness biaswas-7.34%-2.28%. Matrix effect factor was 0.93-1.05. ConclusionThe method established is sensitive, accurate and stable, satisfying the quantification requirements of imatinib mesylate in plasma, and it could be used in clinical analysis.
作者
茆勇
游庆军
姚鲁帅
盛青松
华东
Mao Yong;You Qingjun;Yao Lushuai;Sheng Qingsong;Hua Dong(Department of Oncology,Affiliated Hospital of Jiangnan University,Wuxi 214062,China;Wuxi Zenzer Precision Clinical Laboratories,INC,Wuxi 214174,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第10期1838-1840,共3页
Chinese Journal of Experimental Surgery
关键词
甲磺酸伊马替尼
液相色谱-串联质谱法
性能验证
Imatinib mesylate
Liquid chromatography tandem mass spectrometry
Method validation
