摘要
目的利用体外模型评价纳米氧化铜(CuO-NPs)对气血屏障(ACB)通透性的影响。方法粒径为40、80和100 nm的近球型CuO-NPs用于试验。采用肺毛细血管内皮细胞(PCECs)和A549细胞共培养于细胞插板底膜(A549和PCECs分别生长于底膜内外表面)的方式建立气血屏障通透性测试模型,以跨内皮细胞电阻(TEER)评估模型的屏障特点。利用模型进行三种粒径CuO-NPs通透性影响试验,并计算通透系数(Pe)用以表示气血屏障通透性改变大小。结果建立了气血屏障通透性影响测试模型。所建立模型TEER峰值为(114.8±8.7)Ω/cm^2。通透性影响试验测得40、80和100 nm CuONPs对模型Pe分别是(1.82±0.21)×10^(-3)、(1.26±0.19)×10^(-3)和(1.06±0.16)×10^(-3) cm/min,其中40 nm CuO-NPs Pe值高于模型对照组Pe值[(0.96±0.12)×10^(-3) cm/min]。结论拥有小粒经和大比表面积的40 nm CuO-NPs较80和100 nm CuO-NPs对气血屏障通透性作用明显。
Objective To understand the impact of copper oxide nanoparticles (CuO-NPs) on the permeability of alveolarcapillary barrier (ACB) in vitro. Methods 40,80 and 100 nm-sized CuO-NPs with a globoid shape were made to be used in the present study. ACB permeability test model was built up through co-culture of pulmonary capillary endothelial cells (PCECs) with A549 cells in transparent hanging cell insert (A549 cells and PCECs grew on the internal and outside surface separately). The barrier characteristics of model were evaluated by transendothelial electrical resistance (TEER). Next,the permeability impact test was implemented. The model was exposed to CuO-NPs (40,80 and 100 nm) for 60 min, respectively. The permeability coefficient (Pe) of them was calculated to investigate the permeability change. Results ACB permeability test model was built up,the peak TEER value of it was (114.8±8.7)■/cm2. The Pe values of CuO-NPs (40,80 and 100 nm) were calculated as ( 1.82±0.21 )×l0 -3,( 1.26±0.19)×l0-3 and (1.06±0.16)×l0-3 cm/min respectively. Among them, Pe value of CuO-NPs (40 nm) was significantly higher than that of the control group [(0.96±0.12)xl0-3 cm/min]. Conclusion In contrast with 80 and 100 nm-sized CuO -N Ps,40 nm CuO-NPs with smaller size and larger specific surface area may impact ACB permeability in vitro more obviously.
作者
赵康峰
宋予晴
侯智全
顾雯
张宏伟
戴洪兴
鲁波
白雪涛
ZHAO Kang-feng;SONG Yu-qing;HOU Zhi-quan;GU Wen;ZHANG Hong-wei;DAI Hong-xing;LU Bo;BAI Xue-tao(National Institute o f Environmental Health, Chinese Center for Disease Control and Prevention, Beijing 100021, China)
出处
《环境与健康杂志》
CAS
北大核心
2019年第3期189-193,F0003,共6页
Journal of Environment and Health
基金
国家自然科学基金(81372949)
中国疾病预防控制中心青年科研基金资助(2016A206)
关键词
氧化铜纳米颗粒
气血屏障通透性
体外模型
通透性改变
评估
Copper oxide nanoparticles ( CuO -N Ps)
Permeability of alveolar -capillary barrier
In vitro model
Permeability change
Assessment
