摘要
目的探讨组织蛋白酶B(Cathepsin B,Cat B)在肿瘤坏死因子相关凋亡配体(TRAIL)诱导胃腺癌SGC-7901细胞凋亡的作用。方法采用MTT法检测不同浓度TRAIL对胃腺癌SGC-7901细胞增殖情况的影响;用不同浓度CatB抑制剂和TRAIL共同诱导SGC-7901细胞48h,用流式细胞仪检测细胞凋亡率;用不同浓度CatB抑制剂和TRAIL共同诱导SGC-7901细胞48h,用免疫印迹法(Westernblot)检测CatB表达的变化。结果TRAIL对SGC-7901细胞增殖有抑制作用,最佳作用浓度为100μg/L;分别用0μmol/L、0.2μmol/L、0.5μmol/L、1.0μmol/L、2.0μmol/L、4.0μmol/LCA-074(Me)作用时,细胞凋亡率分别为36%、34%、28%、20%、18%、15%(P<0.05),CatB抑制剂浓度越高,细胞凋亡率越低;Westernblot结果表明,CatB表达分别为(1.25±0.08)、(1.14±0.09)、(0.93±0.05)、(0.61±0.07)、(0.38±0.06)和(0.31±0.44)(P<0.05),CatB抑制剂浓度越高,CatB表达越少。结论组织蛋白酶B在TRAIL诱导胃腺癌SGC-7901细胞凋亡中具有调控作用。
Objective To investigate the effect of cathepsin B (Ca B) on apoptosis of gastric adenocarcinoma SGC-7901 cells induced by tumor necrosis factor-related apoptosis ligand (TRAIL). Methods The effects of different concentrations of TRAIL on the proliferation of gastric adenocarcinoma SGC-7901 cells were detected by MTT assay.SGC-7901 cells were induced with different concentrations of Cat B inhibitor and TRAIL for 48 h,and the apoptosis rate was detected by flow cytometry.SGC-7901 cells were induced by Cat B inhibitor and TRAIL for 48 h,and the expression of Cat B was detected by Western blot. Results TRAIL inhibited the proliferation of SGC-7901 cells at an optimal concentration of 100μg/L;0μmol/L,0.2μmol/L,0.5μmol/L,1.0μmol/L,2.0μmol/L,4.0μmol/LCA,respectively.When -074(Me),the apoptotic rate was 36%,34%,28%,20%,18%,15%( P <0.05).The higher the concentration of Cat B inhibitor,the higher the apoptosis rate.Low;Western blot results showed that Cat B expression was (1.25±0.08),(1.14±0.09),(0.93±0.05),(0.61±0.07),(0.38±0.06) and (0.31±0.44)( P <0.05),the higher the concentration of Cat B inhibitor,the less Cat B expression. Conclusion Cathepsin B has a regulatory role in TRAIL-induced apoptosis of gastric adenocarcinoma SGC-7901 cells.
作者
涂龙霞
查道德
李蓓莉
TU Long-xia(Huaibei Vacational and Technical College,Huaibei 235000,China)
出处
《牡丹江医学院学报》
2019年第5期9-11,46,共4页
Journal of Mudanjiang Medical University
基金
安徽省高校自然科学研究重点课题(KJ2018A0712)
